Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves
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概要
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A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7,and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the Nterminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31,227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57% to 84% identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Kariu T
Kyushu Univ. Fukuoka Jpn
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Kariu Tohru
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Shimokawa H
Laboratory Of Biology Fukuoka Dental College
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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SANO Kazunari
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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SHIMOKAWA Hidetoshi
Laboratory of Biology, Fukuoka Dental College
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ITOH Riyoko
Laboratory of Biology, Fukuoka Dental College
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Itoh Riyoko
Laboratory Of Biology Fukuoka Dental College
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Sano Kazunari
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kimura Makoto
Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric
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KIMURA Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN
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