Morphology, host range and phylogenetic diversity of Sphingomonas phages in the floodwater of a Japanese paddy field(Soil Biology)

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概要

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• Members of the Sphingomonas-related genera (Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis) are dominant in bacterial isolates from the floodwater of Japanese paddy fields. Fifty-eight Sphingomonas/Novosphingobium bacteriophages (phages) were isolated to elucidate their morphology, host range and phylogenetic diversity based on the capsid gene (g23) sequence. All of the phages were siphoviruses with isometric or elongated, icosahedral capsids and a long, non-contractile tail. The genomes were double-stranded DNA measuring either 40, 60, 100 or 160kb. The host range of the phages was examined by infecting 16 bacterial isolates from the floodwater, belonging to Sphingomonas, Novosphingobium, Sphingopyxis and Porphyrobacter. The host range was widely different and varied between infection of only the host used for isolation and infection of hosts belonging to the three genera of Sphingomonas, Novosphingobium and Porphyrobacter. All phages had g23, indicating the ubiquity of the g23 gene among Myoviridae and Siphoviridae members. Every g23 sequence of the phages belonged to one of the six uncharacterized Paddy Groups proposed by Fujii et al. (2008). The g23 sequences were identical at the nucleotide level for several phages with isometric and elongated capsids with 60 and 160kb genomes, and between some phages and the clones that were retrieved from distant paddy fields. This indicates the common occurrence of horizontal transfer of g23 in the paddy fields. The g23 sequence does not correlate with the host range of those phages. In addition, a larger degree of divergence of g23 from coliphage T4 in paddy fields compared to marine environments was estimated from the present study.

• 社団法人日本土壌肥料学会の論文

著者

• 木村 真人

  Laboratory Of Soil Biology And Chemistry School Of Agricultural Sciences Nagoya University

• 木村 眞人

  名大農

• Asakawa Susumu

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Asakawa Susumu

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bio-agricultural Sciences Nagoya Univers

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Kimura Makoto

  Nagoya Univ. Aichi Jpn

• Asakawa Susumu

  Nagoya Univ. Nagoya Jpn

• Asakawa S

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Asakawa Susumu

  Graduate School Of Bioagricultural Sciences Nagoya University

• Asakawa S

  Kyushu National Agricultural Experiment Station

• Katoh Masahiko

  Graduate School Of Bioagricultural Sciences Nagoya University:(present Address)zen-noh Agricultural

• Kimura M

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Kimura Makoto

  School Of Agriculture Nagoya University

• Kimura M

  School Of Agriculture Nagoya University

• NAKAYAMA Natsuko

  Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer

• TSUGE Takashi

  Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer

• Nakayama Natsuko

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Tsuge Takashi

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Kimura Makoto

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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