Molecular analysis of the ammonia oxidizing bacterial community in the surface soil layer of a Japanese paddy field(Soil Biology)

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概要

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• Ammonia oxidation to nitrite is the key part in the nitrification-denitrification process that occurs in the oxic-anoxic interface of paddy fields and is, therefore, an important component of the nitrogen cycle. Ammonia oxidation is carried out by autotrophic ammonia oxidizing bacteria (AOB). In this study, we investigated the AOB community in the surface paddy soil layer using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequencing methods using amoA-specific primers targeting a partial stretch of the gene that encodes ammonia monooxygenase A (amoA). DGGE bands with eight different levels of mobility were obtained from the surface soil. The DNA sequences of five bands were identified. All DNA sequences were closely related to Nitrosospira spp. and no Nitrosomonas-like AOB were identified. The deduced amino acid sequences of the five retrieved DGGE bands were closely related (99-100%) to previously published amoA amino acid sequences of the cultured strains Nitrosospira Ka3 and Nitrosospira CT2F. A phylogenetic tree based on the amoA amino acid sequence revealed that all five AOB sequences were grouped within the amoA cluster 1.

• 社団法人日本土壌肥料学会の論文

著者

• 木村 真人

  Laboratory Of Soil Biology And Chemistry School Of Agricultural Sciences Nagoya University

• Asakawa Susumu

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Asakawa Susumu

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bio-agricultural Sciences Nagoya Univers

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• Kimura Makoto

  Nagoya Univ. Aichi Jpn

• Asakawa S

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Asakawa S

  Kyushu National Agricultural Experiment Station

• JIA Zhongjun

  名古屋大院生命農

• Katoh Masahiko

  Graduate School Of Bioagricultural Sciences Nagoya University:(present Address)zen-noh Agricultural

• Kimura M

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Nakajima Yasunori

  Laboratory of Crop Science, Aichi-ken Anjo Research and Extension Center

• Kimura M

  School Of Agriculture Nagoya University

• Jia Zhong‐jun

  Nagoya Univ. Nagoya Jpn

• BOWATTE Saman

  Laboratory of Soil Biology and Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya Unive

• Bowatte Saman

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bio-agricultural Sciences Nagoya Univers

• ISHIHARA Rie

  Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer

• ISHIHARA Rie

  Graduate School of Bioagricultural Sciences, Nagoya University

• Jia Zhong-jun

  Graduate School Of Bioagricultural Sciences Nagoya University:(present Office)max-planck-institute F

• Ishihara Rie

  Graduate School Of Bioagricultural Sciences Nagoya University

• JIA Zhongjun

  Laboratory of Soil Biology and Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya Unive

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Nakajima Yasunori

  Laboratory Of Crop Science Aichi Anjo Research And Extension Center

• Kimura Makoto

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• Ishihara Rie

  Laboratory Of Animal Breeding And Genetics Graduate School Of Agricultural Science Kobe University

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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