Vertical Changes in Bacterial Communities in Percolating Water of a Japanese Paddy Field as Revealed by PCR-DGGE (Soil Biology)

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概要

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• Percolating water was sampled from the plow layer and subsoil layer in a Japanese paddy field, and the bacterial communities were compared together with floodwater by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting a partial 16S rRNA gene and subsequent sequencing. The number of DGGE bands ranged from 16 to 28 with no significant differences among the sampling sites and times. Only 2 bands were common for the three sources of water samples. DGGE bands specific for the floodwater samples and percolating water samples from the plow layer were identified, while percolating water samples from the subsoil layer did not show specific bands but displayed common bands to those of the floodwater samples (7 bands) and percolating water samples from the plow layer (1 band). Cluster analysis of the DGGE banding patterns showed a distinct clustering in the samples of percolating water from the plow layer and a closer relationship between the others. These results suggest that the bacterial communities in percolating water changed during downward movement through the plow layer and subsoil layer. Sequences of the DGGE bands specific for the samples of percolating water from the plow layer showed a close relationship with anaerobic bacteria such as iron-reducers or uncultured bacterial DNA isolated from environments that are considered to be less oxic. On the other hand, the sequences of the bands specific for the samples of floodwater and percolating water from the subsoil layer showed a close relationship with uncultured bacterial DNA isolated from freshwater environments.

• 社団法人日本土壌肥料学会の論文

著者

• KIMURA Makoto

  Graduate School of Bioagricultural Sciences, Nagoya University

• 木村 真人

  Laboratory Of Soil Biology And Chemistry School Of Agricultural Sciences Nagoya University

• Kimura Makoto

  Nagoya Univ. Aichi Jpn

• Shimizu Miho

  Graduate School of Information Sciences and Technology, The University of Tokyo

• Shimizu Miho

  Graduate School Of Bioagricultural Sciences Nagoya University

• MURASE Jun

  Graduate School of Bioagricultural Sciences, Nagoya University

• Matsuya Kazuo

  Laboratory of Crop Science, Aichi-ken Anjo Research and Extension Center

• Hayashi Motoki

  Laboratory of Crop Science, Aichi-ken Anjo Research and Extension Center

• Hayashi Motoki

  Aichi-ken Anjo Research and Extension Center

• Katoh Masahiko

  Graduate School Of Bioagricultural Sciences Nagoya University:(present Address)zen-noh Agricultural

• Kimura M

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Murase Jun

  Graduate School Of Bioagricultural Sciences Nagoya University

• Murase J

  Graduate School Of Bioagricultural Sciences Nagoya University

• Mtsuya Kazuo

  Laboratory Of Crop Science Aichi-ken Anjo Research And Extension Center

• Matsuya Kazuo

  Laboratory Of Crop Science Aichi-ken Anjo Research And Extension Center

• Hayashi Motoki

  Laboratory Of Crop Science Aichi-ken Anjo Research And Extension Center

• Murase J

  Nagoya Univ. Nagoya Jpn

• Kimura Makoto

  Graduate School Of Bioagricultural Sciences Nagoya University

• Kimura Makoto

  Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi

• Kimura Makoto

  Graduate School Of Agricultural Sciences Nagoya University

• Murase Jun

  Graduate School of Bioagricultural Science, Nagoya University

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