Microbiological Factors Affecting the Colonization of Tomato Roots by Ralstonia solanacearum YU1Rif43lux
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概要
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We investigated the effect of prior colonization of sterile soils by a range of soil microbes on the growth of Ralstonia solanacearum YU1Rif43 in an earlier report (Soil Sci. Plant Nutr., 46, 449-459, 2000). Here, we report the effect of prior colonization of tomato roots, the host plant of the pathogen, by soil microbes on succeeding colonization by the pathogen. When the soil microbial community was introduced onto tomato roots, subsequent colonization by YU1Rif43lux, a lux-marked mutant of YU1Rif43, was remarkably suppressed compared with that in the absence of microorganisms which had previously colonized roots (hereafter referred to as priorcolonists). Soil bacteria, rather than fungi, were mainly responsible for the suppression. Next, the effect of individual microbial strains on the colonization by YU1Rif43lux was examined. Some strains did not show any significant suppressive effect on subsequent colonization by YU1Rif43lux, unlike others. All the R. solanacearum strains used in this study, especially R. solanacearum NPS1 (a weakly virulent mutant of YU1), were effective priorcolonists against colonization by YU1Rif43lux, that is, they markedly suppressed subsequent colonization by YU1Rif43lux. Most of the strains that were effective priorcolonists in soil in the earlier report were also effective at suppressing the colonization of tomato roots by YU1Rif43lux. YU1Rif43lux competed on tomato roots with the strains that did not show previously an effect against colonization by YU1Rif43lux, while root colonization by YU1Rif43lux was reduced by co-inoculation with the strains that previously showed acolonization effect. These results suggested that soil bacteria were likely to be responsible for the suppression of colonization by YU1Rif43lux of tomato roots, and that strains belonging to R. solanacearum among individual microbial strains might be the most effective antagonists against colonization by YU1Rif43lux. Using Tn5-induced derivatives of a strain, Pseudomonas fluorescens MelRC2, one of the most effective priorcolonists, the contribution of motility, antibiosis, rhizosphere competence to the suppressiveness of P. fluorescens MelRC2 against colonization by YU1Rif43lux were examined.
著者
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Toyota Koki
Laboratory Of Soil Biology And Chemistry Graduate School Bioagricultural Sciences Nagoya University
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Toyota Koki
Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Kinoshita Tadataka
Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Kimura Makoto
Laboratory Of Soil Biology And Chemistry Graduate School Of Bioagricultural Sciences Nagoya Universi
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Kimura Makoto
Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric
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Kinoshita Tadataka
Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University:(Present addresses)Aichi Prefecture Agricultural Research Center
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KIMURA Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN
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