Thermodynamic Analysis of a Multifunctional RNA-Binding Protein, PhoRpp38, in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
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概要
- 論文の詳細を見る
The protein component PhoRpp38 of Pyrococcus horikoshii ribonuclease P (RNase P) is known to be a multifunctional RNA-binding protein. Previous biochemical data indicate that it binds to two stem-loops in RNase P RNA (PhopRNA). Thermodynamic analysis revealed that PhoRpp38 and PhopRNA interact with each other with an association constant (Ka) of 1.56 × 107 M−1. It was further found that PhoRpp38 simultaneously binds two stem-loop structures in PhopRNA with approximately equal affinity. Crystals of PhoRpp38 in complex with the stem-loop were grown and diffracted to a resolution of 7.0 Å on a synchrotron X-ray source.
- 2012-06-23
著者
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Kakuta Yoshimitsu
Lab. Of Biochemistry Dep. Of Bioscience And Biotechnology Graduate School Fac. Of Agriculture Kyushu
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Kakuta Yoshimitsu
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu
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Kakuta Yoshimitsu
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Nakashima Takashi
Lab. Of Biochemistry Dep. Of Bioscience And Biotechnology Graduate School Fac. Of Agriculture Kyushu
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Nakashima Takashi
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu
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Kimura Makoto
Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric
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Tsumoto Kouhei
Medical Proteomics Laboratory Institute Of Medical Science The University Of Tokyo
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OSHIMA Kosuke
Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University
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Oshima Kosuke
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agriculture Kyushu University
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agriculture Kyushu University
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KIMURA Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN
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