Immunological Characterization of Honey Proteins and Identification of MRJP 1 as an IgE-Binding Protein
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概要
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We encountered a fourth case of honey allergy in Japan. We characterized and identified the IgE-binding proteins in honey using the serum of a honey-allergenic patient. Immunoblot analysis revealed that IgE in the patient serum specifically bound to four proteins in each honey sample. At least three of these IgE-binding proteins were N-linked glycoproteins. To identify the 60-kDa IgE-binding protein in dandelion honey, the N-terminal sequences of the fragmented protein were analyzed, revealing the protein to be major royal jelly protein 1 (MRJP 1). Three IgE-binding proteins removed of N-linked oligosaccharide showed a large reduction in IgE-binding activity as compared with the intact protein. This suggests that the carbohydrates in the IgE-binding proteins are a major epitope for patient IgE.
- 2011-03-23
著者
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HAYASHI Takeshi
Department of Neurology, Okayama University School of Medicine
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Nakashima Takashi
Laboratory Of Structural Biology Graduate School Of Systems Life Sciences Kyushu University
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Arita Takashi
Ehime Seikyou Hospital
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TAKAMATSU Nobue
Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu University
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Nakashima Takashi
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu
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Hayashi Takeshi
Department Of Food And Fermentation Science Faculty Of Food And Nutrition Beppu University
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Hayashi Takeshi
Department Of Computer Science And Systems Engineering Graduate School Of Engineering Kobe Universit
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Takamatsu Nobue
Department Of Food And Fermentation Science Faculty Of Food And Nutrition Beppu University
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Takashi ARITA
Ehime Seikyou Hospital
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