The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3

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概要

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• We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

• 2011-04-23

著者

• Terada Atsushi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• YAMAGUCHI Hiroki

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of

• Nakashima Takashi

  Laboratory Of Structural Biology Graduate School Of Systems Life Sciences Kyushu University

• Kimura Makoto

  Lab. Of Structural Biology Graduate School Of Systems Life Sciences Kyushu Univ.

• Kakuta Yoshimitsu

  Lab. Of Biochemistry Dep. Of Bioscience And Biotechnology Graduate School Fac. Of Agriculture Kyushu

• Kakuta Yoshimitsu

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu

• Kakuta Yoshimitsu

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Nakashima Takashi

  Lab. Of Biochemistry Dep. Of Bioscience And Biotechnology Graduate School Fac. Of Agriculture Kyushu

• Terada Atsushi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Hara Tadashi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Nakashima Takashi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Yamaguchi Hiroki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agriculture Kyushu University

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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