Molecular Cloning of cDNAs Encoding Ribonuclease-related Proteins in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-infection(Biochemistry & Molecular Biology)

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概要

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• We earlier isolated a cDNA clone (NGR1) encoding a wound-inducible ribonuclease (RNase NW) from leaves of Nicotiana glutinosa [Kariu et al. Biosci. Biotechnol. Biochem., 62, 1144-1151 (1998)]. In this study, two distinct cDNA clones, NGR2 and NGR3, encoding proteins with a ribonuclease-related sequence in the N. glutinosa leaves, were amplified and sequenced. The nucleotide sequences of NGR2 and NGR3 consist of 1244 bp and 1069 bp, and have open reading frames encoding 277 (RNase NGR2) and 236 (RNase NGR3) amino acid residues, respectively. The deduced amino acid sequences of the putative RNases NGR2 and NGR3 showed 33% and 58% amino acid sequence identity, respectively, with that of RNase NW and 32% identity with each other. Sequence comparison showed that NGR2 is similar to RNase RNS2 (61%) from Arabidopsis thaliana, while NGR3 is related to RNase LX (84%) from tomato (Lycopersicon esculentum). RNA gel blot analysis showed that the RNase NGR2 gene is constitutively expressed to measurable levels ; it is not increased by either wounding or TMV infection. In contrast, the expression of the NGR3 gene is induced after 48 h upon TMV infection.

• 社団法人日本農芸化学会の論文
• 2002-02-23

著者

• KIMURA Makoto

  Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University

• KARIU Tohru

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• Kawano S

  Kaneka Corp. Hyogo Jpn

• Kawano Shin

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kariu Tohru

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kariu Tohru

  Laboratory Of Biochemistyr Department Of Bioscience And Biotechnology Faculty Of Agriculture Gradaut

• Kawano Shin

  Laboratory Of Biochemistyr Department Of Bioscience And Biotechnology Faculty Of Agriculture Gradaut

• KURATA Naomi

  Laboratory of Biochemistyr, Department of Bioscience and Biotechnology, Faculty of Agriculture, Grad

• Kurata Naomi

  Laboratory Of Biochemistyr Department Of Bioscience And Biotechnology Faculty Of Agriculture Gradaut

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Kimura Makoto

  Laboratory Of Biochemistyr Department Of Bioscience And Biotechnology Faculty Of Agriculture Gradaut

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

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