Microbial Enantioselective Reduction of Acetylpyridine Derivatives(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
The microbial enantioselective reduction of acetylpyridine derivatives was studied. Many microorganisms were found to reduce 5-acetylfuro [2, 3-c] pyridine (AFP) to (S) -5- (1-hydroxyethyl) furo [2, 3-c] -pyridme (FPH). Candida maris IFO10003 reduced AFP to (R) -FPH with high enantioselectivity. The microbial reduction reaction was optimized. The aeration conditions and glucose concentration affected the yield and stereoselectivity. The cells accumulated 17.5 g/l (107 mM) of (R) -FPH with a 99% yield and 97% enantiomeric excess (e. e.). A cell-free extract of C. maris accumulated 91.5 g/l (559 mM) with over 99% e. e. with enzymatic NADH regeneration. (R) -FPH is an important intermediate for the synthesis of HIV reverse-transcriptase inhibitor, and other optically active 1- (pyridyl) ethanol derivatives are versatile chiral building blocks for asymmetric synthesis.
- 社団法人日本農芸化学会の論文
- 2003-04-23
著者
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Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Hasegawa Junzo
Fine Chemicals Research Laboratories Kaneka Corporation
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YASOHARA Yoshihiko
Fine Chemical Research Laboratories, Kaneka Corporation
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KAWANO Shigeru
Fine Chemicals Research Laboratories, Kaneka Corporation
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HORIKAWA Miho
Fine Chemicals Research Laboratories, Kaneka Corporation
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Yasohara Y
Fine Chemicals Research Laboratories Kaneka Corporation
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Yasohara Yoshihiko
Fine Chemicals Research Laboratories
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Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
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Hasegawa Junzo
Life Science R & D Center Kaneka Co.
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Kawano S
Kaneka Corp. Hyogo Jpn
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Horikawa Miho
Fine Chemicals Research Laboratories Kaneka Corporation
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Kawano Shigeru
Fine Chemicals Research Laboratories Kaneka Corporation
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Yasohara Y
Frontier Biochemical And Medicinal Research Laboratories Kaneka Corporation
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KAWANO Shigeru
Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation
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