Cloning and sequencing of a gene encoding of aldehyde oxidase in Pseudomonas sp. AIU 362(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We have cloned a gene encoding an aldehyde oxidase (ALOD) oxidized glyoxal but not glyoxylic acid from Pseudomonas sp. AIU 362. The ALOD gene contained an open reading frame consisting of 888 nucleotides corresponding to 295 amino acid residues. The deduced amino acid sequence exhibited a high similarity to those of 3-hydroxyisobutyrate dehydrogenases (3-HIBDHs). We expressed the cloned gene as an active product in Escherichia coli BL21 cells. The productivity (total units per culture broth volume) of the recombinant ALOD expressed in E. coli BL21 was 20,000-fold higher than that of ALOD in Pseudomonas sp. AIU 362. The recombinant ALOD exhibited ALOD activity and 3-HIBDH activity. The 3-HIBDH from Pseudomonas putida KT2440 also exhibited ALOD activity. Thus, the ALOD from Pseudomonas sp. AIU 362 and 3-HIBDH from P. putida KT2440 were classified into the same enzyme group.
著者
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Sasaki Yasutaka
The United Graduate School of Agricultural Sciences, Iwate University
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Ogawa Jun
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Iwasaki Akira
Life Science R & D Center, Kaneka Co.
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Hasegawa Junzo
Life Science R & D Center, Kaneka Co.
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Isobe Kimiyasu
The United Graduate School of Agricultural Sciences, Iwate University
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清水 昌
京都大学大学院農学研究科
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清水 昌
近畿大学 農
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Kataoka Michihiko
Div. Of Applied Life Sci. Graduate School Of Agriculture Kyoto Univ.
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Kataoka Michihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Isobe Kimiyasu
The United Graduate School Of Agricultural Sciences Iwate University
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Urano Nobuyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Sasaki Yasutaka
The United Graduate School Of Agricultural Sciences Iwate University
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Shimazu S
Div. Appl. Life Sci. Grad. Sch. Agric. Kyoto Univ.
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Shimizu S
Agricultural Sciences Kyoto University
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Shimizu S
Kyoto Univ. Kyoto Jpn
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Shimizu Sakayu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Iwasaki Akira
Life Science R & D Center Kaneka Co.
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Ogawa Jun
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Shiozaki Shozo
Department Of Agricultural Chemistry Kyoto University:(present Office)life Science Institute Nippon
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Kataoka Michihiko
Divisin Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefecture
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Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
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Hasegawa Junzo
Life Science R & D Center Kaneka Co.
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清水 晶
京都大学 大学院
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Shimizu Sakayu
Laboratory Of Fermentation Physiology And Applied Microbiology Division Of Applied Life Sciences Gra
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清水 昌
京都大学応用生命科学専攻
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Kataoka Michihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University:Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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