Purification and Characterization of a Novel (S)-Enantioselective Transaminase from Pseudomonas fluorescens KNK08-18 for the Synthesis of Optically Active Amines
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概要
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Pseudomonas fluorescens KNK08-18, showing (S)-selective transaminase activity, was isolated from soil by an enrichment culture method using (S)-7-methoxy-2-aminotetraline as the main nitrogen source. A transaminase was purified from the strain to homogeneity in seven steps. The relative mass of the enzyme was estimated to be 53 kDa on SDS-polyacrylamide gel electrophoresis and 120 kDa by gel filtration, suggesting a homodimeric structure. The optimal pH and temperature for enzyme activity were about 8.0–8.5 and 40 °C. The purified enzyme produced (S)-7-methoxy-2-aminotetraline, (S)-SMA, from 7-methoxy-2-tetralone (SMT) with high enantioselectivity. Although (S)-1-phenylethylamine was the best amino donor, β-alanine and 4-aminobutyric acid, which are good substrates for typical ω-amino acid transaminase (EC 2.6.1.18) and GABA transaminase (2.6.1.19), were not reacted. It aminated a broad range of carbonyl compounds containing aromatic, non-aromatic, and acidic and non-acidic substrates.
- 2011-11-23
著者
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Hasegawa J
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Yasohara Y
Fine Chemicals Research Laboratories Kaneka Corporation
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Yasohara Yoshihiko
Fine Chemicals Research Laboratories
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Hasegawa J
Fine Chemicals Research Laboratories Kaneka Corporation
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Hasegawa Junzo
Life Science R & D Center Kaneka Co.
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Kawano S
Kaneka Corp. Hyogo Jpn
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Kawano Shigeru
Fine Chemicals Research Laboratories Kaneka Corporation
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Kawano Shigeru
Frontier Biochemical And Medicinal Res. Laboratories Kaneka Corp.
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HASEGAWA Junzo
Frontier Biochemical and Medicinal Research Laboratories, Kaneka Corporation
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YASOHARA Yoshihiko
Frontier Biochemical and Medicinal Research Laboratories, Kaneka Corporation
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ITO Noriyuki
Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation
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Ito Noriyuki
Frontier Biochemical And Medical Research Laboratories Kaneka Corporation
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HASEGAWA Junzo
Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation
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YASOHARA Yoshihiko
Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation
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KAWANO Shigeru
Frontier Biochemical and Medical Research Laboratories, Kaneka Corporation
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