Complete Amino Acid Sequences of Three Proteinase Inhibitors from White Sword Bean (Canavalia gladiata)

スポンサーリンク

概要

• 論文の詳細を見る

• Three major serine proteinase inhibitors (SBI-1,-2,and -3) were purified from the seeds of white sword bean (Canavalia gladiata) by FPLC and reversed-phase HPLC. The sequences of these inhibitors were established by automatic Edman degradation and TOF-mass spectrometry. SBI-1,-2,and -3 consisted of 72,73,and 75 amino acid residues, with molecular masses of 7806.5,7919.8,and 8163.4,respectively. The sequences of SBI-1 and -2 coincided with those of CLT I and II [Terada et al. (1994) Biosci. Biotech. Biochem., 58,376-379] except only N-or C-terminal amino acid residues. Analysis of the amino acid sequences showed that the active sites of the inhibitors contained a Lys^<21>-Ser^<22> against trypsin and Leu^<48>-Ser^<49> against chymotrypsin, respectively. Further, it became apparent that about seven disulfide bonds were present. These results suggest that sword bean inhibitors are members of the Bowman-Birk proteinase inhibitor family.

• 社団法人日本農芸化学会の論文
• 2000-10-23

著者

• OHBA HIDEKI

  Materials Chemistry Department, Kyushu National Industrial Research Institute

• Ohba Hideki

  Materials Chemistry Department Kyushu National Industrial Research Institute

• Sumi Toshihisa

  Materials Chemistry Department Kyushu National Industrial Research Institute

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• PARK SungSoo

  Materials Chemistry Department, Kyushu National Industrial Research Institute

• NAKAMURA Osamu

  Materials Chemistry Department, Kyushu National Industrial Research Institute

• Park Sungsoo

  Materials Chemistry Department Kyushu National Industrial Research Institute

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Nakamura Osamu

  Materials Chemistry Department Kyushu National Industrial Research Institute

• Kimura Makoto

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric

• Park Sung-Soo

  Materials Chemistry Department, Kyushu National Industrial Research Institute

• KIMURA Makoto

  Laboratory for Remediation Research, Plant Science Center, RIKEN

関連論文

• Production of Acid-Stable α-Amylase by Aspergillus kawachii during Barley Shochu-Koji Production
• Crystal Structure of hyperthermophilic Archaeal Initiation Factor 5A : A Homologue of Eukaryotic Initiation Factor 5A (eIF-5A)
• The Structure of the Archaebacterial Ribosomal Protein S7 and Its Possible Interaction with 16S rRNA^1
• Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata
• Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins(Biochemistry & Molecular Biology)
• Proteome Analysis of Wheat Lemma(Biochemistry & Molecular Biology)
• Cytotoxicity Induced by Erythrina variegata Serine Proteinase Inhibitors in Tumor Hematopoietic Stem Cell Lines
• Methanogenic archaeal communities developed in paddy fields in the Kojima Bay polder, estimated by denaturing gradient gel electrophoresis, real-time PCR and sequencing analyses(Soil Biology)
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddles : IV. Effect of Water Percolation on the Binding Type of Humus in a Submerged Plow Layer Soil
• Comparison of Humus Composition in the Subsoil of Japanese Paddy and Upland Fields
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : III. Changes in Binding Type of Humus in Submerged Plow Layer Soil
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : II. Effects of Subsoil Constituents and Drainage on the Accumulation of Organic Matter Leached from the Plow Layer Soil
• Origin and Properties of Humus in the Subsoil of Irrigated Rice Paddies : I. Leaching of Organic Matter from Plow Layer Soil and Accumulation in Subsoil
• Genomic Cloning of Ribonucleases in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-Infection, and Characterization of Their Promoters(Biochemistry & Molecular Biology)
• Contribution of Conserved Asn Residues to the Inhibitory Activities of Kunitz-Type Protease Inhibitors from Plants
• Protein Engineering of Novel Proteinase Inhibitors and Their Effects on the Growth of Spodoptera exigua Larvae(Biochemistry & Molecular Biology)
• Calcium Ions Stabilize a Protein Structure of Hemolytic Lectin CEL-III from Marine Invertebrate Cucumaria echinata
• Molecular Cloning and Functional Expression of cDNA Encoding the Cysteine Proteinase Inhibitor Sca from Sunflower Seeds
• Conformation of the Primary Binding Loop Folded through an Itramolecular Iteraction Contributes to the Strong Chymotrpsin Inhibitory Activity of the Chymotrypsin Inhibitor from Erythrina variegata Seeds
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding a Cysteine Proteinase Inhibitor from Sunflower Seeds
• Chymotrypsin Inhibitor from Erythrina variegata Seeds : Involvement of Amino Acid Residues within the Primary Binding Loop in Potent Inhibitory Activity toward Chymotrypsin
• Cloning, Expression, and Mutagenesis of Trypsin Inhibitor ETIb from Erythrina variegata Seeds
• The Tissue-Type Plasminogen Acitvator Inhibitor ETIa from Erythrina variegata: Structural Basis for the Inhibitory Activity by Cloning, Expression, and Mutagenesis of the cDNA Encoding ETIa
• Molecular Cloning of cDNA That Encodes Chymotrypsin Inhibitor ECI from Erythrina variegata Seeds and Its Expression in Escherichia coli
• Inhibitory Potency of Erythrina variegata Proteinase Inhibitors toward Serine Proteinases in the Blood Coagulation and Fibrinolytic Systems
• Purification, Characterization, and Sequencing of Two Cysteine Proteinase Inhibitors, Sca and Scb, from Sunflower (Helianthus annuus) Seeds
• Amino Acid Sequence of Chymotrypsin Inhibitor ECI from the Seeds of Erythrina variegata (LINN.) var. Orientalis
• Isolation and Primary Structure of Proteinase Inhibitors from Erythrina variegata (LINN.) var. Orientalis Seeds
• On the Interaction of Ribosomal Protein L5 with 5S rRNA(Biochemistry & Molecular Biology)
• Arginine-55 in the β-Arm is Essential for the Activity of DNA-Binding Protein HU from Bacillus stearothermophilus
• Contribution of a Salt Bridge to the Thermostability of DNA Binding Proten HU from Bacillus stearothermophilus Determined by Site-Directed Mutagenesis
• Characterization of the Archaeal Ribonuclease P Proteins from Pyrococcus horikoshii OT3
• Effect of elevated atmospheric CO_2 concentration on ammonia oxidizing bacteria communities inhabiting in rice roots(Soil Biology)
• A Wheat Xylanase Inhibitor Gene, Xip-I, but Not Taxi-I, Is Significantly Induced by Biotic and Abiotic Signals That Trigger Plant Defense
• New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat
• Biotransformation of the Mycotoxin, Zearalenone, to a Non-estrogenic Compound by a Fungal Strain of Clonostachys sp.(Organic Chemistry)
• Structural Evidence for Endocrine Disruptor Bisphenol A Binding to Human Nuclear Receptor ERRγ
• Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli
• The Evolution of the Phenylacetic Acid Degradation Pathway in Bacteria
• Vertical changes in bacterial and archaeal communities with soil depth in Japanese paddy fields(Soil Biology)
• Molecular characterization of methane-oxidizing bacteria associated with rice straw decomposition in a rice field(Soil Biology)
• Primary Structure of 6.5k-Arginine/Glutamate-rich Polypeptide from the Seeds of Sponge Gourd (Luffa cylindrica)
• Microbiological Factors Affecting the Colonization of Tomato Roots by Ralstonia solanacearum YU1Rif43lux
• Suppression of Ralstonia solanacearum in Soil Following Colonization by Other Strains of R. solanacearum
• Morphology, host range and phylogenetic diversity of Sphingomonas phages in the floodwater of a Japanese paddy field(Soil Biology)
• Abundance of bacteriophages of common heterotrophic bacteria in the floodwater of a Japanese paddy field(Soil Biology)
• Seasonal variations in the abundance of virus-like particles and bacteria in the floodwater of a Japanese paddy field(Soil Biology)
• Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2(Biochemistry & Molecular Biology)
• 5-8 Distribution of Photosynthetically Fixed C in Irrigated Rice : I. Contribution to Soil Dissolved Organic C and Microbial Biomass
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : IV. Influence of Rice Straw Incorporated during the Previous Cropping Period
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : III. Effect of Incorporation Site of Rice Straw on CH_4 Emission Rates and Their Variation among Shoots of a Rice Plant
• Methane Production and Its Fate in Paddy Fields : V. Fate of Methane Remaining in Paddy Soil at Harvesting Stage
• Activities of Soil Enzymes in Different Land-Use Systems in Middle Terrace Areas of Lampung Province, South Sumatra, Indonesia
• Contribution of Gln9 and Phe8O to Substrate Binding in Ribonuclease MC1 from Bitter Gourd Seeds
• Amino Acids Conserved at the C-Terminal Half of the Ribonuclease T2 Family Contribute to Protein Stability of the Enzymes
• Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd
• Contribution of the Second OB Fold of Ribosomal Protein S1 from Escherichia coli to the Recognition of TmRNA
• Activities of Some Soil Enzymes in Different Land Use Systems after Deforestation in Hilly Areas of West Lampung, South Sumatra, Indonesia
• Molecular analysis of the ammonia oxidizing bacterial community in the surface soil layer of a Japanese paddy field(Soil Biology)
• Observation of Microbial Colonization on the Surface of Rice Roots along with Their Development and Degradation
• Comparison of microbial community structure between soils suppressive and conducive to Fusarium-wilt of radish
• Epibiotic Microorganisms Associated with Microcrustaceans in Overlying Water of Philippine Paddy Fields
• Impact of Fumigation with Metam Sodium upon Soil Microbial Community Structure in Two Japanese Soils
• Seasonal Variation of Microcrustaceans and Microbial Flora on Their Surface in the Overlying Water of a Japanese Paddy Field
• Epibiotic Bacteria Associated with Microcrustaceans in the Overlying Water of Paddy Fields
• Isolation and Characterization of Bacteria Responsible for the Suppression of Fusarium oxysporum f. sp. raphani on the Host Rhizoplane
• Mechanisms of Suppression of Fusarium oxysporum f. sp. raphani in Soils So-Called Suppressive to Fusarium-Wilt of Radish
• Molecular Cloning of cDNAs Encoding Ribonuclease-related Proteins in Nicotiana glutinosa Leaves, as Induced in Response to Wounding or to TMV-infection(Biochemistry & Molecular Biology)
• Expression of Nicotiana glutinosa Ribonucleases in Escherichia coli(Biochemistry & Molecular Biology)
• Crystal Structures of the Nicotiana glutinosa Ribonuclease NT in Complex with Nucleoside Monophosphates
• The Action of Rice Branching Enzyme I (BEI) on Starches
• Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) from Oryza sativa L
• The C-Terminal Portion of an Archaeal Toxin, aRelE, Plays a Crucial Role in Protein Synthesis Inhibition
• Crystal Structure and Functional Analysis of an Archaeal Chromatin Protein Alba from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Phenotypic Characterization of a Mutant of Burkholderia cepacia MRT11 Defective in Rhizosphere Competence
• Effects of Soil Temperature and Anaerobiosis on Degradation of Biodegradable Plastics in Soil and Their Degrading Microorganisms
• Characterization of ammonia oxidizing bacteria associated with weeds in a Japanese paddy field using amoA gene fragments(Soil Biology)
• 2P-019 マウス硫酸転移酵素SULT5A1のX線結晶構造解析(蛋白質・構造(2),第46回日本生物物理学会年会)
• The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3
• The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus precatorius
• Isolation and Molecular Characterization of Four Arginine/Glutamate Rich Polypeptides from the Seeds of Sponge Gourd (Luffa cylindrica)
• Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : II. Contribution of Organic Constituents in Rice Straw
• Effect of Rice Straw Application on CH_4 Emission from Paddy Fields : I. Effect of Weathering of Rice Straw in the Field during Off-Crop Season
• Primary Structure of a Cysteine Proteinase Inhibitor from the Fruit of Avocado (Persea americana Mill)
• Isolation and Characterization of a Wound-inducible Ribonuclease from Nicotiana glutinosa Leaves
• Molecular analysis of fungal communities of biodegradable plastics in two Japanese soils(Soil Biology)
• Complete Amino Acid Sequences of Three Proteinase Inhibitors from White Sword Bean (Canavalia gladiata)
• In Vitro Phosphorylation of Initiation Factor 2α (aIF2α) from Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Archaeal Homologs of Human RNase P Protein Pairs Pop5 with Rpp30 and Rpp21 with Rpp29 Work on Distinct Functional Domains of the RNA Subunit
• Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Protein–Protein Interactions in the Subunits of Ribonuclease P in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Water-Soluble Organic Materials in Paddy Soil Ecosystem : I. Fractionation of Water-Soluble Organic Materials in Leachate from Submerged Paddy Soils Using PVP and Ion Exchange Resins
• The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana)(Biological Chemistry)
• Methane Production and Its Fate in Paddy Fields VIII. Seasonal Variations in the Amount of Methane Retained in Soil
• Carbon flow into ammonia-oxidizing bacteria and archaea during decomposition of ^C-labeled plant residues in soil(Soil biology)
• Comparison of microbial community structure between soils suppressive and conducive to Fusarium-wilt of radish
• Thermodynamic Analysis of a Multifunctional RNA-Binding Protein, PhoRpp38, in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Bacterial consortia in iron-deposited colonies formed on paddy soil surface under microaerobic conditions(Soil biology)

もっと見る 閉じる

スポンサーリンク