Spectroscopic Analysis of the Cytoagglutinating Activity of Abrin-b Isolated from Abrus precatorius Seeds against Leukemic Cells
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概要
- 論文の詳細を見る
The cytoagglutinating activity of abrin-b, a toxic lectin isolated from Abrus precatorius seeds, against cultured cell strains derived from accute lymphoblast leukemia (ALL) was investigated by visible (VIS) spectroscopy. Upon addition of abrin-b, the turbidity at 600 nm of cell suspension decreased and this change could be recorded as the cytoagglutination curve. From this curve, the cytoag-glutination velocity (CV) and cytoagglutination intensity (CI) of each cell strain was measured. Each cell strain showed the respective CV and CI values and the cell strains derived from the T cell line were strongly agglutinated by abrin-b compared with those derived from the B cell line. Further, it has become apparent that the cytoagglutinating activity increased with an increase in the order of the differentiation of cell strains.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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ITOH Kyogo
Departments of Immunology, Kurume University School of Medicine
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Ohba Hideki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Itoh K
Univ. Tokyo Tokyo Jpn
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HOSHINO Tomoaki
Department of Immunology, Kurume University School of Medicine
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Itoh Kyogo
Department Of Immunology
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OHBA Hideki
Kyushu National Industrial Research Institute
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TOYOKAWA Tetsuya
Kyushu National Industrial Research Institute
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YASUDA Seiji
Kyushu National Industrial Research Institute
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Yasuda S
Kyushu National Industrial Research Institute
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Ohba H
Kyushu National Industrial Res. Inst. Saga Jpn
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Yasuda Syouhei
Graduate School Of Science And Engineering Waseda University
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Yasuda Shoji
Laboratory Of Protein Chemistry And Engineering Graduate School Of Genetic Resources Technology Kyus
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Hoshino Tomoaki
Department Of Immunology Kurume University School Of Medicine
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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