Reduction of the Immunogenicity of β-Lactoglobulin from Cow's Milk by Conjugation with a Dextran Derivative

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概要

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• β-Lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow’s milk, and some immunological properties of the conjugate (DG-BLG) were studied. The conjugate was prepared by modifying BLG with DG-ONSu and purified in a Sephadex G-100 column. The analytical data for DG-BLG indicated that 5.2 moles of DG-ONSu with a mean molecular weight of 9,300 were covalently attached to the amino groups of the BLG molecule. Conjugation with DG-ONSu greatly decreased the reactivity of BLG with anti-BLG antibodies and suppressed their production in vivo due to its shielding action for epitope(s) on the protein’s molecular surface. It was also found that DG-BLG was resistant to proteolytic enzymes. These findings allow us to suggest that DG-ONSu could be advantageously used to suppress the hypersensitivity mediated by IgG antibodies in milk allergy.

• 社団法人 日本農芸化学会の論文
• 2010-04-23

著者

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Yamasaki N

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• FUKASAWA Masashi

  Department of AIDS Research, Institute of Tropical Medicine, Nagasaki University

• SAKAKIBARA Ryuzo

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• FUKUMOTO Shoichi

  Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of

• NODAKE Yuichi

  Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International University

• Nodake Yuichi

  Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern

• Fukumoto Shoichi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Sakakibara Ryuzo

  Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern

• Sakakibara Ryuzo

  Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University

• Fukasawa Masashi

  Department Of Aids Research Institute Of Tropical Medicine Nagasaki University

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

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