Biochemical Studies on Oral Toxicity of Ricin. IV. A Fate of Orally Administered Ricin in Rats

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概要

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• After oral administration of ricin in rats, its distribution in the gastrointestinal tract, body fluids and principal organs was determined by an enzyme immunoassay, and the immunoreactive ricin detected was identified by gel filtration followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, protein blotting and the immunobinding method. When ricin D (10mg/kg rat) was given orally to a rat, which dose is equivalent to 1/3 LD_<50>, about 75% of the ricin was found in the stomach and small intestine within 2h, and most of it was transferred to the large intestine after 24h. It was also demonstrated by an in vitro toxicity test of immunoreactive ricin in the blood and lymph obtained from the intoxicated rats that a part of the ricin was absorbed from the small intestine into the tissues and organs via the circulatory systems (lymphatic and blood vessels) as the active ricin. The participation of the blood vessels was greater in the absorption of ricin from the gastrointestinal tract than that of the lymphatic system. Ricin, after absorption, was detected in liver and spleen and ricin found in the liver was predominantly in the form of intact ricin, although an undetectable amount of ricin in other organs cannot be eliminated. These results infer that a small fraction of orally-given ricin was transferred to the circulating system and was responsible for rat's death as in the case of i.p. administration.

• 公益社団法人日本薬学会の論文

著者

• Tanabe Shinzo

  Department Of Analytical Chemistry Meiji College Of Pharmacy:faculty Of Pharmaceutical Sciences Chib

• ISHIGURO Masatsune

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• TANABE Shuichi

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• MATORI Yumiko

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• SAKAKIBARA Ryuzo

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• Ishiguro M

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• Ishiguro Masatsune

  Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University

• Tanabe Shuichi

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• Matori Yumiko

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• Sakakibara Ryuzo

  Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University

• Sakakibara Ryuzo

  Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University

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