Biochemical Studies on Oral Toxicity of Racin. V. The Role of Lectin Activity in the Intestinal Absorption of Ricin

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概要

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• In order to investigate a possible role of lectin activity of ricin in its absorption from the small intestine, we prepared two ricin derivatives, BMH-ricin, prepared by crosslinking A and B chains of ricin with 1,6-bismaleimidohexane, was nearly non-toxic but the lectin activity was unaltered. And, NBS-ricin, prepared by the oxidation of tryptophanyl residues of ricin with N-bromosuccinimide, was not only non-toxic but also non-lectinic.After the oral administration of ricin derivatives to rats, their interaction with the digestive tract and absorption into the circulatory systems have been compared with those of ricin, immunochemically and histologically. It was shown by immunostaining that ricin and BMH-ricin could bind to the intestinal mucosa, whereas NBS-ricin could not. No appreciable damage in the small intestine from rats treated with either BMH-ricin or NBS-ricin has been observed, in contrast to ricin treatment where severe impairment of the small intestinal tissues resulted after 5 h. Immunoreactive ricin in the liver has been determined with the ricin enzyme immunoassay (EIA). When compared at 48 h after oral administration, NBS-ricin was not detected, whereas BMH-ricin was found to be 38 μg/liver and ricin 100 μg/liver.From these results, it was inferred that the lectin activity of ricin plays an important role in the absorption of ricin from the small intestine and that the absorption of ricin protein was enhanced by its high toxicity.

• 社団法人日本薬学会の論文
• 1992-05-25

著者

• 榊原 隆三

  長崎大・薬

• 川瀬 義久

  Department Of Pathology The Atomic Disease Institute School Of Medicine Nagasaki University

• 石黒 正恒

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 真鳥 由美子

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 田辺 修一

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 関根 一郎

  Department of Pathology, The Atomic Disease Institute, School of Medicine, Nagasaki University

• 榊原 隆三

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 石黒 正恒

  長崎大学薬学部

• 関根 一郎

  Department Of Pathology The Atomic Disease Institute School Of Medicine Nagasaki University

• 石黒 正恒

  Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University

• 田辺 修一

  長崎大学 薬 生化

• Ishiguro Masatsune

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• Ishiguro Masatsune

  Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University

• 真鳥 由美子

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

関連論文

• Biochemical Studies on Oral Toxicity of Racin. V. The Role of Lectin Activity in the Intestinal Absorption of Ricin
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• タイトル無し
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