Effective Purification of Human Chorionic Gonadotropin and Its Subunits From Pregnant Women's Urinary Peptides Adsorbed on Reverse-Phase Resin

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概要

• 論文の詳細を見る

• Human chorionic gonadotropin (hCG) was extracted and purified by reverse-phase resin (Sepralyte C_8 resin) adsorption and Sephadex G-100 column chromatography from urine of pregnant women. Approximately 15000 IU of hCG was recovered from 1000ml of urine by C_8 rsin adsorption. hCG from the gel-filtration step stimulated testosterone production in rat Leydig cells and had a specific activity of approximately 8000 IU/mg, a value which was higher than those of commercial hCGs. Furthermore, hCG purified to near-homogeneity was separated effectively into its subunits by reverse-phase high-performance liquid chromatography using an acetonitrile gradient in the presence of 0.1% trifluoroacetic acid with no special pretreatment for dissociation of subunits. The separated subunits were able to re-associate. The techniques used are simple and may be suitable for not only laboratory but also industrial production of hCG and its subunits.

• 社団法人日本薬学会の論文
• 1990-05-25

著者

• 境 晶子

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• 榊原 隆三

  長崎大・薬

• 石黒 正恒

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 榊原 隆三

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 石黒 正恒

  長崎大学薬学部

• 冨永 伸明

  長崎大・薬

• 宮崎 修一

  長崎大学薬学部薬品生物化学

• 重村 俊明

  長崎大学薬学部薬品生物化学

• 宮崎 修一

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 重村 俊明

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 冨永 伸明

  Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University

• 石黒 正恒

  Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University

• Ishiguro Masatsune

  Department Of Biochemistry School Of Clinical Pharmaceutical Sciences Nagasaki University

• Ishiguro Masatsune

  Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University

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