Human Placental Fructose-6-phosphate, 2-kinase/Fructose-2, 6-bisphosphatase : Its Isozymic Form, Expression and Characterization
スポンサーリンク
概要
- 論文の詳細を見る
The nucleotide sequence of 1981 bp cDNA containing the entire coding region of a human placental fructose-6-phosphate, 2-kinase/fructose-2, 6-bisphosphatase was determined. The sequence encodes 469 amino acids and, based on homology to the rat testis enzyme, appears to be the testis-type isozyme expressed in placenta. The enzyme was expressed in Escherichia coli BL21 (DE3) by using a T7 RNA polymerase-based expression system and purified to homogeneity. The expressed enzyme was bifunctional with specific activities of 75 and 80 mU/mg of kinase and phosphatase, respectively. Kinetic parameters of the expressed enzyme are similar to those of the rat testis enzyme.
- 社団法人日本農芸化学会の論文
- 1997-11-23
著者
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HIRATA Takafumi
Department of Earth and Planetary Sciences, Graduate School of Science and Technology, Tokyo Institu
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Hirata Takafumi
Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University
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SAKAKIBARA Ryuzo
Department of Biochemistry, School of Clinical Pharmaceutical Sciences, Nagasaki University
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UEMURA Mika
Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University
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OKAMURA Noriko
Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University
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KATO Mie
Department of Protozoology, Institute of Tropical Medicine, Nagasaki University
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Uemura Mika
Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University
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Okamura N
Ehime Coll. Health Sci. Ehime Jpn
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Okamura Noriko
Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University
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Kato Mie
Department Of Biochemistry School Of Pharmaceutical Sciences Nagasaki University
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Sakakibara R
Kyushu Women's Univ. Fukuoka Jpn
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Sakakibara Ryuzo
Department Of Biochemistry School Of Clinical Pharmaceutical Science Nagasaki University
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