Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori

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概要

• 論文の詳細を見る

• Chymotrypsin inhibitors were purified from larval hemolymph of Bombyx mori. The purification steps involved ammonium sulfate fractionation (30-70%) and liquid chromatography on Butyl-Toyopearl 650M, Sephadex G-75, concanavalin A-Sepharose, Mono-Q, and Mono-S columns. Fourteen isoforms of chymotrypsin inhibitor were resolved in hybrid crosses : ten isoforms from c60 × n511, eight from k03 × FJ1, and nine from i10 × p50. Chromatographic and electrophoretic studies on the inhibitors suggested that they were expressed co-dominantly and could be classified into three molecular-size groups : 8-13 kDa, 40 kDa, and 42kDa. CI-b4, CI-13a, CI-13b, and CI-13c in the first group, CI-3 in the second group, and CI-8 in the third group were present in all of the hybrids. Inhibitors in the third size group had an affinity for concanavalin A-Sepharose. It was found that CI-13 was composed of three different inhibitors.

• 社団法人日本農芸化学会の論文
• 1993-07-23

著者

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Shirai K

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• ASO yoichi

  Laboratory of Protein Chemistry & Engineering, Kyushu University

• SHIRAI Koji

  Laboratory of Silkworm Physiology, Department of Applied Biology, Faculty of Textile Science and Tec

• Fujii Hiroshi

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• Shirai Koji

  Laboratory Of Insect Genetic Resources Graduate School Of Genetic Resources And Technology Kyushu Un

• Aso Yoichi

  Laboratory Of Protein Chemistry And Engineering Genetic Resources Graduate School Of Genetic Resourc

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Fujii H

  Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Shinohara Tatsumi

  Laboratory of Protein Chemistry and Engineering Genetic Resources, Graduate School of Genetic Resour

• Shinohara Tatsumi

  Laboratory Of Protein Chemistry And Engineering Genetic Resources Graduate School Of Genetic Resourc

• Funatsu Gunki

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Genetic Resources And Technology

• ASO Yoichi

  Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

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