Development of the Irradiation Method for the First Instar Silkworm Larvae Using Locally Targeted Heavy-ion Microbeam

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概要

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• To carry out the radio-microsurgery study using silkworm, Bombyx mori, we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-μm-diameter microbeam of 220 MeV carbon (^<12>C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6% of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.

• 日本放射線影響学会の論文
• 2007-05-15

著者

• Satoh Katsuya

  日本原子力研究所

• TATEI Kazuaki

  Dept. of Biochem., Gunma Univ. Graduate Sch. Med.

• Kinashi Yuko

  Radiation Safety And Control Research Reactor Institute Kyoto University

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)

• SAKASHITA Tetsuya

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• Shirai K

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Wada Seiichi

  Department Of Veterinary Medicine Kitasato University Graduate School Of Veterinary Medicine And Ani

• Wada Seiichi

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Kakizaki Takehiko

  Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.

• Funayama Tomoo

  Microbeam Radiation Biology Group Quantum Beam Science Directorate Japan Atomic Energy Agency

• Funayama Tomoo

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• KAGAWA Yasuhiro

  The Institute of Physical and Chemical Research

• FUKAMOTO Kana

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• KAKIZAKI Takehiko

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• SHIRAI Koji

  Laboratory of Silkworm Physiology, Department of Applied Biology, Faculty of Textile Science and Tec

• KIGUCHI Kenji

  Laboratory of Silkworm Physiology, Department of Applied Biology, Faculty of Textile Science and Tec

• Kanai T

  National Institute Of Radiological Sciences

• SAKATA Toshiyuki

  Laboratory of Clinical Chemistry, National Cardiovascular Center

• Hamada Nobuyuki

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Kobayashi Yasuhiko

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Shirai Katsuyuki

  Department Of Radiation Oncology Gunma University Graduate School Of Medicine

• Sakashita Tetsuya

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Shirai Koji

  Laboratory Of Insect Genetic Resources Graduate School Of Genetic Resources And Technology Kyushu Un

• Kiguchi Kenji

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kiguchi Kenji

  Laboratory Of Developmental Biology Department Of Insect Genetics And Breeding National Institute Of

• Fukamoto Kana

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kaminuma Takuya

  Department Of Radiation Oncology Gunma University Graduate School Of Medicine

• Satoh K

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Furusawa T

  Microbeam Radiation Biology Group Radiation-applied Biology Division Quantum Beam Science Directorat

• Shimura Sachiko

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kimura Takafumi

  Graduate School Of Life Sciences Tohoku Univ.

• Kanai Tatsuaki

  Div. Acce. Res. Natl. Inst. Radiol Sci.

• Kiguchi K

  Faculty Of Textile Science And Technology Shinshu University

• Sakata Toshiyuki

  Laboratory Of Clinical Chemistry National Cardiovascular Center

• Kakizaki Takehiko

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Kanai Tatsuaki

  Department Of Technical Support And Development Fundamental Technology Center National Institute Of

• Kakizaki Takehiko

  Dep. Of Radiation Oncology Gunma Univ. Graduate School Of Medicine

• Suzuki Keiji

  Atomic Bomb Disease Institute Graduate School Of Biomedical Sciences Nagasaki University

• Shibuya Kei

  Dep. Of Radiation Oncology Gunma Univ. Graduate School Of Medicine

• Kaneyasu Tomoko

  Graduate School Of Life Sciences Tohoku University

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