Effectiveness of Carbon-ion Beams for Apoptosis Induction in Rat Primary Immature Hippocampal Neurons
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概要
- 論文の詳細を見る
The direct biological effects of radiation, particularly accelerated heavy particle ions, on neurons are not fully known. Hence, the direct effect of carbon-ion beams on immature neurons was investigated by comparing to the effect of X-rays in vitro using primary hippocampal neurons. Primary neurons were prepared from hippocampi of fetal rats at embryonic day 18 from timed pregnant Wistar rats and cultured with Bankers methods. At 7 Days In Vitro (DIV), the cells were irradiated with 140 kV X-ray and 18.3 MeV/amu carbon-ion beams (LET = 108 keV/μm). The cells were fixed with 4% paraformaldehyde at 12 hours after irradiation. Then, the cells were treated with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and DAPI staining for measuring the percentage of apoptosis (apoptotic index: AI). AI in sham-irradiated hippocampal neurons was 18%. The value of AI (AIs) of the cells irradiated with X-rays at 10 or 30 Gy were 15% or 23%, respectively. AI in cells irradiated with carbon-ion beams at 1 Gy, 3 Gy, 5 Gy and 10 Gy were 22%, 23%, 24% and 33%, respectively. AI was significantly increased by carbon-ion beams at 10 Gy (p < 0.001). The apoptosis of hippocampal neurons increased in a dose-dependent manner following both X-ray and carbon-ion beams irradiation. Carbon-ion beams were about 10-fold more effective than X-rays for apoptosis induction in immature hippocampal neurons.
- Journal of Radiation Research 編集委員会の論文
- 2010-11-16
著者
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NAKANO Takashi
Department of Physics, Kyoto University
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SUZUKI Yoshiyuki
Department of Radiation Oncology, Gunma University Graduate school of Medicine
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TAKAHASHI Takeo
Department of Radiation Oncology, Gunma University Graduate school of Medicine
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TATEI Kazuaki
Dept. of Biochem., Gunma Univ. Graduate Sch. Med.
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YOSHIDA Yukari
Gunma University Heavy Ion Medical Center
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木梨 友子
京都大学原子炉実験所附属粒子線腫瘍学研究センター
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Kinashi Yuko
Radiation Safety And Control Research Reactor Institute Kyoto University
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SHIRAI Katsuyuki
Department of Radiation Oncology, Gunma University Graduate School of Medicine
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NODA Shin-ei
Department of Radiation Oncology, Gunma University Graduate School of Medicine
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FUNAYAMA Tomoo
Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)
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KOBAYASHI Yasuhiko
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
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Shirai K
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Noda Shin-ei
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Nakano T
Centrifuge Project Team Nasda
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Funayama Tomoo
Microbeam Radiation Biology Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Funayama Tomoo
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Nakano Takashi
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Nakano Takashi
Department Of Anatomy Aichi Medical University
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Nakano Takashi
Department Of Radiology And Radiation Oncology Gunma University Graduate School Of Medicine
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Kinashi Yuko
Radiation Oncology Research Laboratory Research Reactor Institute Kyoto University
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KAGAWA Yasuhiro
The Institute of Physical and Chemical Research
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Tamaki Tomoaki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Kobayashi Yasuhiko
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Shirai Katsuyuki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Suzuki Yoshiyuki
Department Of Hepatology Toranomon Hospital
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Suzuki Yoshiyuki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Taketomi Takahashi
Department Of Diagnostic Radiology And Nuclear Medicine Gunma University Graduate School Of Medicine
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Kinashi Yuko
Kyoto Univ. Research Rcactor Institute
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Nonaka Tetsuo
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Nonaka Tetsuo
Department Of Radiology Gunma Cancer Center
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KAMINUMA Takuya
Department of Radiation Oncology, Gunma University Graduate School of Medicine
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MIZUI Toshiyuki
Department of Neurobiology and Behavior, Gunma University Graduate School of Medicine
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SHIRAO Tomoaki
Department of Neurobiology and Behavior, Gunma University Graduate School of Medicine
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Shirao Tomoaki
Department Of Neurobiology And Behavior Gunma University Graduate School Of Medicine
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Kaminuma Takuya
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Mizui Toshiyuki
Department Of Neurobiology And Behavior Gunma University Graduate School Of Medicine
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Takahashi Takeo
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Furusawa T
Microbeam Radiation Biology Group Radiation-applied Biology Division Quantum Beam Science Directorat
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Shibuya Kei
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Tanaka Naritaka
Department Of General Surgical Science (surgery I) Gunma University Graduate School Of Medicine
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Nakazawa Tsugio
Department Of Tumor Pathology Gunma University Graduate School Of Medicine
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Suzuki Keiji
Atomic Bomb Disease Institute Graduate School Of Biomedical Sciences Nagasaki University
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Suzuki Yoshiyuki
Department Of Cardiovascular Medicine Hokkaido University School Of Medicine
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Takahashi Takeo
Department Of Anesthesiology Sapporo Medical College And Hospital
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Takahashi T
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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Shibuya Kei
Dep. Of Radiation Oncology Gunma Univ. Graduate School Of Medicine
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Suzuki Yoshiyuki
Department Of Applied Physics Tohoku University
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Shirai Katsuyuki
Department Of Radiation Oncology Gunma Prefectural Cancer Center
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Kaneyasu Tomoko
Graduate School Of Life Sciences Tohoku University
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Kobayashi Yasuhiko
Advanced Science Research Center Japan Atomic Energy Research Institute
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