Protective Effect of Urinary Trypsin Inhibitor on the Development of Radiation-Induced Lung Fibrosis in Mice
スポンサーリンク
概要
- 論文の詳細を見る
This study aimed to analyze whether Ulinastatin, a urinary trypsin inhibitor (UTI), inhibits the TGF-β signaling pathway and lung fibrosis induced by thoracic irradiation in a lung injury mouse model. The thoraces of 9-week-old female fibrosis-sensitive C57BL/6 mice were irradiated with a single X-ray dose of 12 Gy or 24 Gy. UTI was administrated intraperitoneally at a dose of 200,000 units/kg concurrently with radiation (concurrent UTI) or daily during the post-irradiation period for 8–14 days (post-RT UTI). Mice were sacrificed at 16 weeks after irradiation to assess the histological grade of lung fibrosis and immunohistochemical TGF-β expression. Survival rates of mice given 24 Gy to the whole lung ± UTI were also compared. Post-RT UTI reduced the score of lung fibrosis in mice, but concurrent UTI had no beneficial effects in irradiated mice. The fibrosis score in post-RT UTI mice was 3.2 ± 1.0, which was significantly smaller than that of irradiated mice without UTI treatment (RT alone; 6.0 ± 1.3; p < 0.01). The rates of TGF-β positive cells in post-RT UTI and the RT alone mice were 0.18 ± 0.03 and 0.23 ± 0.04, respectively (p < 0.01). There was a significantly positive correlation between the fibrosis score and the TGF-β positive rate (R2 = 0.26, p < 0.01). The survival rate at 30 weeks for post-RT UTI mice was significantly better than that of RT alone mice (33% vs. 10%, p < 0.05). The administration of post-RT UTI suppressed TGF-β expression and radiation-induced lung fibrosis, which resulted in significant survival prolongation of the irradiated mice.
- 2010-05-16
著者
-
NAKANO Takashi
Department of Physics, Kyoto University
-
HASEGAWA Masatoshi
Department of Radiation Oncology, Nara Medical University
-
OHNO Tatsuya
Department of Radiation Oncology, Gunma University Graduate school of Medicine
-
ISHIKAWA Hitoshi
Department of Radiation Oncology, Gunma University Graduate school of Medicine
-
SUZUKI Yoshiyuki
Department of Radiation Oncology, Gunma University Graduate school of Medicine
-
TAKAHASHI Takeo
Department of Radiation Oncology, Gunma University Graduate school of Medicine
-
Ohno Tatsuya
放射線医学総合研究所重粒子医科学センター病院
-
Ohno Tatsuya
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
KATOH Hiroyuki
Department of Radiation Oncology, Gunma University Graduate School of Medicine
-
YOSHIDA Yukari
Gunma University Heavy Ion Medical Center
-
Nakano T
Centrifuge Project Team Nasda
-
Nakano Takashi
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Nakano Takashi
Department Of Anatomy Aichi Medical University
-
Nakano Takashi
Department Of Radiology And Radiation Oncology Gunma University Graduate School Of Medicine
-
Ikehira H
Department Of Medical Imaging National Institute Of Radiological Sciences
-
Ikehira Hiroo
Division Of Advanced Technology For Medical Imaging Research Center Of Charged Particle Technique Na
-
Hasegawa Masatoshi
Department Of Radiation Oncology Nara Medical University
-
Hasegawa Masatoshi
Department Of Drug Metabolism Research Center Taisho Pharmacetutical Co. Ltd.
-
Tamaki Tomoaki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Kato Hirotoshi
Division Of Radiation Medicine National Institute Of Radiological Sciences
-
Ishikawa Hitoshi
Department Of Orthopedic Surgery Kobe University School Of Medicine
-
Suzuki Yoshiyuki
Department Of Hepatology Toranomon Hospital
-
Suzuki Yoshiyuki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Taketomi Takahashi
Department Of Diagnostic Radiology And Nuclear Medicine Gunma University Graduate School Of Medicine
-
Ishikawa Hitoshi
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Nonaka Tetsuo
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Nonaka Tetsuo
Department Of Radiology Gunma Cancer Center
-
Takahashi Takeo
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Tanaka Naritaka
Department Of General Surgical Science (surgery I) Gunma University Graduate School Of Medicine
-
Katoh Hiroyuki
Department Of Gastroenterology Hokkaido University Graduate School Of Medicine
-
Ohno Tatsuya
Department Of Radiation Oncology Graduate School Of Medicine Gunma University
-
Nakazawa Tsugio
Department Of Tumor Pathology Gunma University Graduate School Of Medicine
-
Suzuki Yoshiyuki
Department Of Cardiovascular Medicine Hokkaido University School Of Medicine
-
Ishikawa Hitoshi
Department Of Mechanical Engineering Tokyo University Of Science
-
Takahashi Takeo
Department Of Anesthesiology Sapporo Medical College And Hospital
-
Hasegawa Masatoshi
Department Of Chemistry Faculty Of Science Toho University
-
Takahashi T
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
Suzuki Yoshiyuki
Department Of Applied Physics Tohoku University
-
Kato Hiroyuki
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
-
HASEGAWA Masatoshi
Department of Applied Chemistry, Faculty of Science, University of Tokyo
-
Ishikawa Hitoshi
Department of Electrical Engineering, College of Science and Technology, Nihon University, Chiyoda, Tokyo 101-8308, Japan
関連論文
- Effective Potential in the Strong-Coupling Lattice QCD with Next-to-Next-to-Leading Order Effects(Nuclear Physics)
- CT-based 3D Dose-Volume Parameter of the Rectum and Late Rectal Complication in Patients with Cervical Cancer Treated with High-Dose-Rate Intracavitary Brachytherapy
- Phantom Study on Radiotherapy Planning Using PET/CT : Delineation of GTV by Evaluation SUV
- Current Advancement in Radiation Therapy for Uterine Cervical Cancer
- Protective Effect of Urinary Trypsin Inhibitor on the Development of Radiation-Induced Lung Fibrosis in Mice
- Efficacy of platinum analogue for advanced hepatocellular carcinoma unresponsive to transcatheter arterial chemoembolization with epirubicin
- The Efficacy of Interferon-beta Monotherapy for Elderly Patients with Type C Hepatitis of Genotype 2
- Potential of laparoscopy in chronic liver disease with hepatitis B and C viruses
- Inhibitory effect of branched-chain amino acid granules on progression of compensated liver cirrhosis due to hepatitis C virus
- Hepatocarcinogenesis Following HCV RNA Eradication by Interferon in Chronic Hepatitis Patients
- The Efficacy of Short-Term Interferon-Beta Therapy for Type C Cirrhotic Patients with Genotype 2a and Low Virus Load
- Factors associated with the virological response of lamivudine-resistant hepatitis B virus during combination therapy with adefovir dipivoxil plus lamivudine
- Interferon lowers tumor recurrence rate after surgical resection or ablation of hepatocellular carcinoma : a pilot study of patients with hepatitis B virus-related cirrhosis
- Efficacy of interferon monotherapy in young adult patients with chronic hepatitis C virus infection
- Cost-effectiveness of radiofrequency ablation and surgical therapy for small hepatocellular carcinoma of 3cm or less in diameter
- Adequate timing of ribavirin reduction in patients with hemolysis during combination therapy of interferon and ribavirin for chronic hepatitis C
- Efficacy of lamivudine treatment in Japanese patients with hepatitis B virus-related cirrhosis
- Efficacy of 6-month interferon therapy in chronic hepatitis B virus infection in Japan
- Infection with hepatitis B virus genotype A in Tokyo, Japan during 1976 through 2001
- Interferon monotherapy for patients with chronic hepatitis C and normal serum aminotransferase levels at commencement of treatment
- Factors contributing of ribavirin dose reduction due to anemia during interferon alfa2b and ribavirin combination therapy for chronic hepatitis C
- Peripheral CD8+/CD25+ lymphocytes may be implicated in hepatocellular injuries in patients with acute-onset autoimmune hepatitis
- Hepatocellular carcinoma in noncirrhotic young adult patients with chronic hepatitis B viral infection
- Influence of Histological Subtype on Survival after Combined Therapy of Surgery and Radiation in WHO Grade 3 Glioma
- Physical and Histopathological Assessment of the Effects of Metallic Stents on Radiation Therapy
- Proliferation and cell death of human glioblastoma cells after carbon-ion beam exposure : Morphologic and morphometric analyses
- Intracavitary Combined with CT-guided Interstitial Brachytherapy for Locally Advanced Uterine Cervical Cancer: Introduction of the Technique and a Case Presentation
- Radiation-induced ICAM-1 Expression via TGF-β1 Pathway on Human Umbilical Vein Endothelial Cells; Comparison between X-ray and Carbon-ion Beam Irradiation
- LATE RECTAL BLEEDING AND GENITOURINARY MORBIDITY AFTER HIGH DOSE RATE BRACHYTHERAPY COMBINED WITH HYPOFRACTIONATED EXTERNAL BEAM RADIOTHERAPY FOR LOCALIZED PROSTATE CANCER
- Nutritional Status of Lactating Mothers and Their Breast Milk Concentration of Iron, Zinc and Copper in Rural Vietnam
- Phosphorous-31 Magnetic Resonance Spectroscopy of Cervical Cancer Using Transvaginal Surface Coil
- Changes in the Pharmacokinetics of Gd-DTPA in Experimental Tumors after Charged Particle Radiation : Comparison with γ-Ray Radiation
- Neural damage due to temporal lobe epilepsy : Dual-nuclei (proton and phosphorus) magnetic resonance spectroscopy study
- ^F-magnetic resonance spectroscopy and chemical shift imaging for schizophrenic patients using haloperidol decanoate
- Comparison of the Radiobiological Effect of Carbon Ion Beam Therapy and Conventional Radiation Therapy on Cervical Cancer
- An improved procedure of electron microscopic in situ hybridization for detecting adenovirus DNA
- Pneumocystis jiroveci pneumonia as an atypical presentation of X-linked agammaglobulinemia
- Deformation capacity of large diameter high strength bolts with waisted shank subjected to axial tensile force
- Apoptosis of hypertrophic chondrocytes in rat cartilaginous growth plate
- Primary Breast Lymphoma Successfully Treated with Combination Therapy Including Local Radiation Therapy: A Report of Two Cases
- The Role of Radiation Therapy for Stage IIIB Non-Small Cell Lung Cancer : Impact of Clinical Nodal Stage on Survival
- Hyperfractionated and Hypofractionated Radiation Therapy for Human Malignant Glioma Xenograft in Nude Mice
- Higher Efficacy of Urinary Bone Resorption Marker Measurements in Assessing Response to Treatment for Osteoporosis in Postmenopausal Women
- Status of Japanese Radiation Oncology
- The measurement of blood flow parameters with deuterium stable isotope MR imaging
- Differences in Metabolic and Morphological Reactions after Radiation Therapy : Proton NMR spectroscopy and Imaging of Patients with Intracranial Tumors
- Technical acquisition and dosimetric assessment of iodine-125 permanent brachytherapy in localized prostate cancer : Our first series of 100 patients
- The Benefit of Small Bowel and Pelvic Bone Sparing in Excluding Common Iliac Lymph Node Region from Conventional Radiation Fields in Patients with Uterine Cervical Cancer: A Dosimetric Study
- Radiation therapy for kaposi's sarcoma associated with acquired immunodeficiency syndrome: Tokyo Metropolitan Komagome Hospital experience
- Growth Cone Collapse and Neurite Retractions : An Approach to Examine X-irradiation Affects on Neuron Cells
- Immunological alterations found in mesothelioma patients and supporting experimental evidence
- p53 Protein Expression and Radiation-Induced Apoptosis in Human Tumors Transplanted to Nude Mice
- Comparison of Primary Thyroid Lymphoma with Anaplastic Thyroid Carcinoma on Computed Tomographic Imaging
- Theory of XPS and BIS Spectra in Ce_2O_3 and CeO_2
- Radiotherapeutic Management of Brain Metastases from Breast Cancer
- Micronucleus Assay of Human Lymphocytes : A Comparison of Cytokinesis-block and Human Capillary Blood Lymphocytes Methods
- Nanotransportation system for cholera toxin in Vibrio cholerae 01
- The Benefit of Small Bowel and Pelvic Bone Sparing in Excluding Common Iliac Lymph Node Region from Conventional Radiation Fields in Patients with Uterine Cervical Cancer : A Dosimetric Study
- Ceramide Induces Myogenic Differentiation and Apoptosis in Drosophila Schneider Cells
- Heavy-ion-induced bystander killing of human lung cancer cells : Role of gap junctional intercellular communication
- Direct visualization and quantification of the anticancer agent, cis-diamminedichloro-platinum(II), in human lung cancer cells using in-air microparticle-induced X-ray emission analysis
- Poly(ester imide)s Possessing Low CTE and Low Water Absorption (II) : Effect of Substituents
- Development of calcium releasing activity induced by inositol trisphosphate and cyclic ADP-ribose during in vitro maturation of sea urchin oocytes
- Geographical Variation in Seed Weight of Plantago asiatica L.
- Endobrouchial metastasis of breast cancer 5 years after breast-conserving therapy
- Effectiveness of Carbon-ion Beams for Apoptosis Induction in Rat Primary Immature Hippocampal Neurons
- Higher pAkt Expression Predicts a Significant Worse Prognosis in Glioblastomas
- Pegylated Interferon-Associated Retinopathy in Chronic Hepatitis C Patients
- Heavy-Ion Microbeam Irradiation Induces Bystander Killing of Human Cells
- A LET-Dependent Decrease in the Apoptotic Response of Normal Human Fibroblast Cultures to Isosurvival Doses of γ-Rays and Energetic Heavy Ions
- Induction of a Critical Elevation of Povidone-Iodine Absorption in the Treatment of a Burn Patient: Report of a Case
- Aggressive clinical course of giant cell tumor arising from thoracic vertebra after a long latent period
- Influence of Gender, Age and Lifestyle Factors on Micronuclei Frequency in Healthy Japanese Populations
- Effect and Mechanism of Betaxolol and Timolol on Vascular Relaxation in Isolated Rabbit Ciliary Artery
- Pupillary function in early-stage presbyopia and effects of an adrenergic agent on accommodation
- Effects of Isopropyl Unoprostone on Rabbit Ciliary Artery
- Presynaptic Effects of Botulinum Toxin Type A on the Neuronally Evoked Response of Albino and Pigmented Rabbit Iris Sphincter and Dilator Muscles
- The Daily Course of Transpiration Rate and Stomatal Conductance of Polygonum cuspidatum in Mt. Fuji.
- Effects of Ocular Dominance and Visual Input on Body Sway
- BACTERIAL DENSITY AND DISTRIBUTIONS OF FLUORIDE AND CALCIUM IN HUMAN DENTAL PLAQUE
- A Multicenter Study on the Prevalence of Psychiatric Disorders among New Referrals for Epilepsy in Japan
- Nuclear Translocation of Gold Labeled-testosterone-bovine Serum Albumin Conjugate through the Nuclear Double Membranes in Rat Spermatids
- Connective tissue configuration in the human liver hilar region with special reference to the liver capsule and vascular sheath
- A New Method for the Synthesis of Baccatin III
- Stereocontrolled Synthesis of the BC Ring System of Taxol
- Exposure of Normal Human Fibroblasts to Heavy-Ion Radiation Promotes Their Morphological Differentiation
- Clinical features of measles in immunocompromised children
- Bipolar Hip Arthroplasty for Osteoarthritis of the Hip Joint
- Secondary Malignant Fibrous Histiocytoma Following Radiation Therapy for Carcinoma of the Uterine Cervix: Report of Two Cases
- Intracavitary Combined with CT-guided Interstitial Brachytherapy for Locally Advanced Uterine Cervical Cancer : Introduction of the Technique and a Case Presentation
- Acute disseminated encephalomyelitis in a boy with elevated plasma levels of thrombin-antithrombin III complex
- An anomalous muscle linking superior and inferior rectus muscles in the orbit
- Posterior Aspect of the Orbital Septum Is Reinforced by Ligaments
- Electrostatic Interactions between Surfactants and Water Soluble Dyes at the Air/Water Interface as Studied by Second Harmonic Interferometry
- Quarkyonic Matter in Lattice QCD at Strong Coupling
- Radioligand Binding Characteristics of the Endothelin Receptor in the Rabbit Iris
- Radiation-induced ICAM-1 Expression via TGF-β1 Pathway on Human Umbilical Vein Endothelial Cells ; Comparison between X-ray and Carbon-ion Beam Irradiation
- Comparison of Chemoradiotherapy with Radiotherapy Alone in Patients with Esophageal Adenocarcinoma
- Expression of Hypoxia-inducible Factor 1α Predicts Clinical Outcome after Preoperative Hyperthermo-chemoradiotherapy for Locally Advanced Rectal Cancer
- Axillary lymph node accumulation on FDG-PET/CT after influenza vaccination