Kinetic Analysis of Double-Strand Break Rejoining Reveals the DNA Reparability of γ-Irradiated Tobacco Cultured Cells
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概要
- 論文の詳細を見る
The rejoining efficiency of double-strand breaks (DSBs) was quantified by a DNA fragment-size analysis in tobacco protoplasts and CHO-K1 cells following γ-ray irradiation in order to compare DNA reparability of higher plants with mammals. Results showed that the DSB rejoining efficiency of tobacco protoplasts is dependent on the temperature of post-irradiation cultivation and that it reaches a maximum at 27°C, which represents the most suitable temperature for protoplast cultivation. The DSB rejoining kinetics of tobacco protoplasts were well represented by a biphasic-exponential equation: half of initial-induced DSBs were rejoined for 1 h and the others were almost rejoined within 4 h. We found that the DSB rejoining kinetics of tobacco protoplasts at 27°C are the same as those of CHO-K1 cells at 37°C. These findings indicate that the DSB rejoining efficiency of tobacco protoplasts and CHO-K1 cells are comparable at their respective cell cultivation temperatures, suggesting that DSB rejoining efficiency is little responsible for the higher radiation-tolerance of tobacco protoplasts.
- Journal of Radiation Research 編集委員会の論文
- 2009-03-16
著者
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木梨 友子
京都大学原子炉実験所附属粒子線腫瘍学研究センター
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Kinashi Yuko
Radiation Safety And Control Research Reactor Institute Kyoto University
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Tanaka Atsushi
Quantum Beam Sci. Directorate Japan Atomic Energy Agency
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Wada Seiichi
Department Of Veterinary Medicine Kitasato University Graduate School Of Veterinary Medicine And Ani
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Narumi Issay
Quantum Beam Sci. Directorate Japan Atomic Energy Agency
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Funayama Tomoo
Microbeam Radiation Biology Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Hase Yoshihiro
Quantum Beam Science Directorate Japan Atomic Energy Agency
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Kinashi Yuko
Radiation Oncology Research Laboratory Research Reactor Institute Kyoto University
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KAGAWA Yasuhiro
The Institute of Physical and Chemical Research
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Tanaka Atushi
Quantum Beam Science Directorate Japan Atomic Energy Agency
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Kinashi Yuko
Kyoto Univ. Research Rcactor Institute
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FUNAYAMA Tomoo
Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency
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Furusawa T
Microbeam Radiation Biology Group Radiation-applied Biology Division Quantum Beam Science Directorat
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HASE Yoshihiro
Radiation-Applied Biology Division, Japan Atomic Energy Agency
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TANAKA Atsushi
Radiation-Applied Biology Division, Japan Atomic Energy Agency
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YOKOTA Yuichiro
Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency
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KOBAYASHI Yasuhiko
Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency
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NARUMI Isaay
Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency
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Narumi Isaay
Radiation-applied Biology Division Quantum Beam Science Directorate Japan Atomic Energy Agency
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Yokota Yuichiro
Radiation-applied Biology Division Quantum Beam Science Directorate Japan Atomic Energy Agency
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Hase Yoshihiro
Radiation-applied Biology Division Qubs Jaea
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Tanaka Atsushi
Radiation-applied Biology Division Japan Atomic Energy Agency
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Hase Yoshihiro
Radiation-applied Biology Division Japan Atomic Energy Agency
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Kobayashi Yasuhiko
Advanced Science Research Center Japan Atomic Energy Research Institute
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