Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes
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概要
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We have examined in vitro radiosensitivities and radioresponses to ^<60>Co γ-rays irradiation in feline T-lymphocyte cell lines, FeT-J and FL-4. There seemed to be no significant difference in clonogenic survival between the two lines. The mean lethal dose for both was both 1.9 Gy, and surviving fraction at 2 Gy was 0.30 and 0.48 for FeT-J and FL-4 cells, respectively. However, TUNEL assay indicated much higher degrees of apoptosis induction in FeT-J cells (>40%) than in FL-4 cells (<10%) at 4 days after 15 Gy irradiation. Microscopic examination revealed a larger population of multi-nucleate cells in FL-4 cells (60.3%) than in FeT-J cells (16.0%) at 4 days after 15 Gy irradiation, suggesting that a larger ratio of mitotic catastrophe occurred in FL-4 cells. These results suggest that FeT-J is more likely to be induced into apoptosis and FL-4 is more likely to fall into mitotic catastrophe, and eventually necrosis; both of them showed a similar surviving fraction against γ-rays. The results also indicate that FL-4 cells follow a process other than apoptosis to cell death, suggesting the presence of a regulatory mechanism that may control the relationship between mitotic catastrophe and apoptosis in feline T-lymphocytes.
- 日本放射線影響学会の論文
著者
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TATEI Kazuaki
Dept. of Biochem., Gunma Univ. Graduate Sch. Med.
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Ishioka Noriaki
奈良県立医科大学 生物学
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Ishioka Noriaki
京都工芸繊維大学 工芸科学部応用生物学
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Kinashi Yuko
Radiation Safety And Control Research Reactor Institute Kyoto University
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FUNAYAMA Tomoo
Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)
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SAKASHITA Tetsuya
Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)
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KOBAYASHI Yasuhiko
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
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Wada Seiichi
Department Of Veterinary Medicine Kitasato University Graduate School Of Veterinary Medicine And Ani
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Wada Seiichi
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Ito Nobuhiko
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Kakizaki Takehiko
Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.
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Funayama Tomoo
Microbeam Radiation Biology Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Funayama Tomoo
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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KAGAWA Yasuhiro
The Institute of Physical and Chemical Research
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HAMADA Nobuyuki
Microbeam Radiation Biology Group, Japan Atomic Energy Agency
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HOHDATSU Tsutomu
Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University
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Kanai T
National Institute Of Radiological Sciences
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Ishioka Noriaki
Department Of Space Environmental Medicine Kagoshima University Graduate School Of Medical And Denta
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Hamada Nobuyuki
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Kobayashi Yasuhiko
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Sakashita Tetsuya
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Ishioka Noriaki
Department Of Molecular Life Science Tokai University School Of Medicine
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Kaminuma Takuya
Department Of Radiation Oncology Gunma University Graduate School Of Medicine
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HARA Takamitsu
Department of Quantum Biology, Gunma University Graduate School of Medicine
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Hattori Tomoyasu
Dep. Of Biochemistry Gunma Univ. Graduate School Of Medicine
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Furusawa T
Microbeam Radiation Biology Group Radiation-applied Biology Division Quantum Beam Science Directorat
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Kimura Takafumi
Graduate School Of Life Sciences Tohoku Univ.
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Kakizaki Takehiko
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Kanai Tatsuaki
Div. Acce. Res. Natl. Inst. Radiol Sci.
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Natsuhori Masahiro
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Hiramitsu Tadahisa
Department Of Quantum Biology Gunma University Graduate School Of Medicine
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SANO Tadashi
Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasat
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Kakizaki Takehiko
Microbeam Radiation Biology Group Japan Atomic Energy Agency
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Kanai Tatsuaki
Department Of Technical Support And Development Fundamental Technology Center National Institute Of
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Kakizaki Takehiko
Dep. Of Radiation Oncology Gunma Univ. Graduate School Of Medicine
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Sano Tadashi
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Hohdatsu Tsutomu
Laboratory Of Veterinary Infectious Disease School Of Veterinary Medicine Kitasato University
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Sano Tadashi
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Natsuhori Masahiro
Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato
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Kaneyasu Tomoko
Graduate School Of Life Sciences Tohoku University
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