Detection of DNA Damage in Individual Cells Induced by Heavy-ion Irradiation with an Non-denaturing Comet Assay

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概要

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• Investigating the biological effects of high-LET heavy-ion irradiation at low fluence is important to evaluate the risk of radiation in space. It is especially necessary to detect radiation damage induced by a precise number of heavy ions in individual cells. We thus compared the number of ions traversing a cell and the DNA damage produced by ion hits. We applied a comet assay to measure the DNA damage in individual cells. Cells attached on ion-track detector (CR-39) were irradiated with 17.3 MeV/u ^<12>C, 15.7 MeV/u, 10.4 MeV/u ^<20>Ne ion and 7.2 MeV/u ^<40>Ar beams at TIARA, JAERI-Takasaki. After irradiation, CR-39 was covered with 1% agarose. The agarose was allowed to solidify on a glass slide, and then the electrophoresis was performed. Afterward, the CR-39 was taken off the glass slide. The agarose gel on the CR-39 was stained with ethidium bromide and the opposite side of the CR-39 was etched with a KOH-ethanol solution at 37 ℃. We observed that heavy ions with higher LET values induced heavier DNA damage, even with the same number of ion hits within the irradiated cells. The result indicated that the amount of DNA damage induced by one particle depended on the LET value of the heavy ions.

• 日本放射線影響学会の論文

著者

• Ito Nobuhiko

  Department of Cardiology, JR Tokyo General Hospital

• Ishioka Noriaki

  奈良県立医科大学 生物学

• Ishioka Noriaki

  京都工芸繊維大学 工芸科学部応用生物学

• WADA Seiichi

  Biotech. Lab., JAERI Takasaki

• FUNAYAMA Tomoo

  Biotech. Lab., JAERI Takasaki

• KOBAYASHI Yasuhiko

  Biotech. Lab., JAERI Takasaki

• Wada Seiichi

  Department Of Veterinary Medicine Kitasato University Graduate School Of Veterinary Medicine And Ani

• Ito Nobuhiko

  Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato

• Ito Nobuhiko

  Department Of Cardiology Jr Tokyo General Hospital

• Yamamoto Kazuo

  東北大学 生命科研究

• YAMAMOTO Kazuo

  Department of Clinical Laboratory Medicine, Shiga University of Medical Science

• Ishioka Noriaki

  Department Of Space Environmental Medicine Kagoshima University Graduate School Of Medical And Denta

• Ishioka Noriaki

  Department Of Molecular Life Science Tokai University School Of Medicine

• Yamamoto Kazuo

  大阪府立大学 生命環境科学部先端病態解析学

• Yamamoto K

  Department Of Molecular Pathology Cancer Research Institute Kanazawa University

• NATSUHORI MASAHIRO

  Department of Veterinary Medicine, Kitasato University

• YAMAMOTO Kazuo

  Kanagawa Institute of Technology

• Furusawa T

  Microbeam Radiation Biology Group Radiation-applied Biology Division Quantum Beam Science Directorat

• Yamamoto K

  Kanagawa Institute Of Technology

• Yamamoto Kazuo

  Department Of Biomolecular Sciences Graduate School Of Life Sciences Tohoku University

• Natsuhori Masahiro

  Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato

• WADA Seiichi

  Biotech Laboratory, JAERI

• KOBAYASHI Yasuhiko

  Biotech Laboratory, JAERI

• Yamamoto Kazuo

  Department of Biology, Chiba University

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