An Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids

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概要

• 論文の詳細を見る

• An improved system to examine forward mutations that occurred in the supF gene of Escherichia coli carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of supF plasmids propagated in wild-type, recA^- and mutM^- mutY^- E. coli strains, the mutation frequencies of which were 1.3×10^-7 , 6.3×10^-7 and 1.5×10^-6 , respectively. Sequence analysis of the supF mutant plasmids revealed that G:C→T:A and G:C→C:G transversions dominated. This improved system allows rapid scoring and sequencing forward mutations in the supF gene, thus permitting its use as a genetic target for repair and mutagenesis studies in bacteria and mammalian cells.

著者

• OBATA FUMIKO

  Biological Institute, Graduate School of Science, Tohoku University

• NUNOSHIBA TATSUO

  Biological Institute, Graduate School of Science, Tohoku University

• HASHIMOTO-GOTOH TAMOTSU

  Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine

• YAMAMOTO KAZUO

  Biological Institute, Graduate School of Science, Tohoku University

• Obata Fumiko

  Biological Institute Graduate School Of Science Tohoku University

• Yamamoto Kazuo

  東北大学 生命科研究

• Nunoshiba Tatsuo

  東北大学 生命科学研究科

• Nunoshiba T

  Dept. Biomol. Sci. Grad. Sch. Life Sci. Tohoku Univ.

• Nunoshiba Tatsuo

  Biological Institute Graduate School Of Science Tohoku University

• Hashimoto-gotoh Tamotsu

  Department Of Biochemistry And Molecular Genetics Kyoto Prefectural University Of Medicine

• Yamamoto Kazuo

  大阪府立大学 生命環境科学部先端病態解析学

• Yamamoto K

  Department Of Molecular Pathology Cancer Research Institute Kanazawa University

• YAMAMOTO Kazuo

  Kanagawa Institute of Technology

• Yamamoto K

  Kanagawa Institute Of Technology

• Hashimoto Gotoh

  Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine

関連論文

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