Mutational Specificity of the Ferrous Ion in a supF Gene of Endonuclease III/VIII Deficient Escherichia coli

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概要

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• When 125 μM Fe^<2+> /EDTA treated plasmid pUB3 was used to transfect an Escherichia coli NKJ2004 (nth nei) host, which is totally defective in glycosylases for thymine glycol and 5-hydroxycytosine, a 3.7 fold increase in mutation frequency was observed. Among 46 supF mutants sequenced, 28 had base substitutions, with G:C→C:G transversion predominant (14 cases), followed by G:C→T:A transversion (6 cases) and G:C→A:T transition (6 cases). The results are consistent with our previous Fe^<2+> mutagenesis results where, in the wild type host, 78% were base substitutions, with G:C→C:G transversion (59%) predominant, followed by G:C→T:A transversion (28%) and G:C→A:T transition (11%). Treatment of pUB3 DNA with Fe^<2+> /EDTA did not yield formation of Endonuclease III sensitive sites. The possibility of 5-hydroxycytosine as the causative lesion for Fe^<2+> induced G:C→C:G transversion is discussed.

• 日本放射線影響学会の論文

著者

• TANO KEIZO

  Research Reactor Institute, Kyoto University

• UTSUMI HIROSHI

  Research Reactor Institute, Kyoto University

• Utsumi Hiroshi

  Research Reactor Institute Kyoto University

• Utsumi Hiroshi

  Health Res. Foundation

• Tano Keizo

  Res. Reactor Inst. Kyoto Univ.

• Tano Keizo

  Particle Radiation Biology Res. Reactor Inst. Kyoto Univ.

• Tano K

  Kyoto Univ. Osaka Jpn

• Tano Kaori

  Kyoto Univ.

• Utsumi Hiroshi

  Kyoto Univ.

• SHIMAMURA HIDEO

  Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University

• AKASAKA SUSUMU

  Osaka Prefectural Institute of Public Health

• KUBO KIHEI

  Department of Radiology, School of Veterinary Medicine, University of Osaka Prefecture

• SAITO YUSUKE

  Biological Institute, Graduate School of Science, Tohoku University

• NAKAJIMA SATOSHI

  Biological Institute, Graduate School of Science, Tohoku University

• UTSUMI HIROSHI

  Biological Institute, Graduate School of Science, Tohoku University

• Yamamoto Kazuo

  東北大学 生命科研究

• Tano Keizo

  Research Reactor Institute Kyoto Univ.

• Yamamoto Kazuo

  大阪府立大学 生命環境科学部先端病態解析学

• Yamamoto K

  Department Of Molecular Pathology Cancer Research Institute Kanazawa University

• YAMAMOTO Kazuo

  Kanagawa Institute of Technology

• Utsumi H

  Research Reactor Institute Kyoto University

• Kubo Kihei

  Department Of Advanced Pathobiology Graduate School Of Life & Environmental Sciences Osaka Prefe

• Kubo Kihei

  Department Of Veterinary Science Graduate School Of Agriculture And Biological Science Osaka Prefect

• Kubo Kihei

  Laboratory Of Veterinary Radiology Department Of Veterinary Medicine College Of Agriculture Osaka Pr

• Saito Y

  Biological Institute Graduate School Of Science Tohoku University

• Akasaka Susumu

  Publ. Health Osaka Prefec.

• Akasaka Susumu

  Osaka Pref. Inst. Pub. Health

• Shimamura Hideo

  Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University

• Shimamura Hideo

  Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University: (present Addres

• Yamamoto K

  Kanagawa Institute Of Technology

• Takimoto Koichi

  Faculty Of Agriculture Yamaguchi University

• Nakajima Satoshi

  Biological Institute Graduate School Of Science Tohoku University

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