Spectrum of Spontaneous Mutations in the Cyclic AMP Receptor Protein Gene on Chromosomal DNA of Escherichia coli
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概要
- 論文の詳細を見る
We determined 46 spontaneous mutations occurring in the cyclic AMP receptor protein gene (crp) on the chromosomal DNA of Escherichia coli by the use of PCR cloning. Of 24 base substitutions, 17 were transversions and 7 transitions including all types of base substitutions. The frequency of the changes of A:T base pairs was similar to that of G:C pairs, suggesting that A:T pair is also a target for base substitution. Frameshifts including seven -1 and four +1 frameshifts occurred at the sites of a run of identical bases. Deletions extending 18 and 172 bases occurred at the sites where the deleted sequences were flanked by short repeated sequences at the junction. The insertions of IS2 element or its inverted sequence were detected in two and six mutations, respectively. The assay system of the mutation used here is available for the determination of the mutational spectrum of base substitutions.
- 日本放射線影響学会の論文
著者
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YAMAMOTO KAZUO
Biological Institute, Graduate School of Science, Tohoku University
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TAKIMOTO KOICHI
Department of Biological Chemistry, Faculty of Agriculture
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TACHIBANA Akira
Radiat. Biol. Cent., Kyoto Univ.
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Yamamoto Kazuo
東北大学 生命科研究
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AYAKI Hitoshi
Radiation Biol. Center, Kyoto Univ.
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AYAKI Hitoshi
Department of Public Health, Kobe University Graduate School of Medicine
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Akira Tachibana
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Department Of Biology Faculty Of Science Osaka City University
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Tachibana Akira
Radiat. Biol. Cent. Kyoto Univ.
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Yamamoto Kazuo
大阪府立大学 生命環境科学部先端病態解析学
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Yamamoto K
Department Of Molecular Pathology Cancer Research Institute Kanazawa University
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YAMAMOTO Kazuo
Kanagawa Institute of Technology
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Ayaki H
Department Of Public Health Kobe University Graduate School Of Medicine
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Ayaki Hitoshi
Radiat. Biol. Center Kyoto Univ.
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Yamamoto K
Kanagawa Institute Of Technology
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Tachibana Akira
Rad. Biol. Canter Kyoto Univ.
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Takimoto K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Takimoto Koichi
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Tachibana Akira
Rad. Biol. Center Kyoto Univ.
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