No Induction of p53 Phosphorylation and Few Focus Formation of Phosphorylated H2AX Suggest Efficient Repair of DNA Damage During Chronic Low-dose-rate Irradiation in Human Cells

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概要

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• Human fibroblast cells obtained from a normal individual and immortalized by introduction of the hTERT gene were irradiated with 0 to 5 Gy of acute high-dose-rate radiation (1.8 Gy/min) or chronic low-dose-rate radiation (0.3 mGy/min) in the G_0 phase, and p53 activation was studied. After high-dose-rate irradiation, a dose-dependent induction of Ser15 phosphorylation was observed, whereas after low-dose-rate irradiation almost none was observed. Then we analyzed the focus formation of phosphorylated histone H2AX protein, which is closely correlated with the induction of double-strand breaks. High-dose-rate radiation induced a significant number of foci in a dose-dependent manner, whereas, low-dose-rate radiation could induce only a few foci even at the highest dose. These results strongly suggest that DNA damage induced by low-dose-rate radiation such as a double-strand break is efficiently repaired during chronic irradiation.

• 日本放射線影響学会の論文

著者

• Komatsu Kenshi

  Radiation Biology Center Kyoto Univ.

• Nakamura H

  Department Of Analytical Chemistry Hoshi University

• Yamane Yoshihiro

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• NAKAMURA Hideaki

  Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute

• YASUI Yoshihiro

  Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute

• ISHIZAKI Kanji

  Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute

• TACHIBANA Akira

  Radiat. Biol. Cent., Kyoto Univ.

• 石崎 寛治

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• Ishizaki Kanji

  Central Laboratory And Radiation Biology Research Institute Aich Cancer Center

• Ishizaki K

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• Ishizaki Kanji

  Laboratory Of Experimental Radiology Aichi Cancer Center

• Hayashi Yuko

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden

• Nakamura Hideaki

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• Nakamura Hideaki

  Department Of Analytical Chemistry Hoshi University

• Nakamura Hideaki

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden

• Ishizaki K

  Aichi Cancer Center Research Institute

• Ishizaki Kanji

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• Ishizaki Kanji

  大阪府立大学先端科学イノベーションセンター 放射線生命科学

• Tachibana Akira

  Radiat. Biol. Cent. Kyoto Univ.

• Hayashi Yuko

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute

• Hayashi Y

  Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden

• Hayashi Yasushi

  Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine

• Nakamura Hajime

  Gastroenterological Surgery Nagoya University Graduate School Of Medicine

• Nakamura Hideaki

  Department of Analytical Chemistry, Hoshi University

• NAKAMURA Hideaki

  Department of Physics, Kumamoto University

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