Establishment of Immortal Normal and Ataxia Telangiectasia Fibroblast Cell Lines by Introduction of the hTERT Gene
スポンサーリンク
概要
- 論文の詳細を見る
To establish immortal human cells, we introduced the human catalytic subunit of telomerase(hTERT) gene into skin fibroblast cells obtained from normal and ataxia telangiectasia(AT) individuals of Japanese origin. After hTERT introduction, these cells continue to grow beyond a population doubling number of 200 while maintaining their original radiosensitivity. Inductions of p53, phosphorylation of Ser15 in p53, and induction of p21 by X-ray irradiation in immortal cells derived from normal individual were not affected by the hTERT introduction. Both normal and AT immortal cells exhibited an apparent inhibition of growth as original primary cells when they reached confluence. Karyotype analysis has revealed that they are in a diploid range. These results suggest that cells immortalized by hTERT introduction retain their original characteristics except for immortalization, and that they may be useful for analyzing various effects of radiation on human cells.
- 日本放射線影響学会の論文
著者
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Nakamura H
Department Of Analytical Chemistry Hoshi University
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NAKAMURA Hideaki
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute
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ISHIZAKI Kanji
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute
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HAMAGUCHI Michinari
Division of Cancer Biology, Nagoya University Graduate School of Medicine
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石崎 寛治
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Ishizaki Kanji
Central Laboratory And Radiation Biology Research Institute Aich Cancer Center
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Ishizaki K
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Ishizaki Kanji
Laboratory Of Experimental Radiology Aichi Cancer Center
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Hayashi Yuko
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden
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Nakamura Hideaki
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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NAKATSUGAWA Shigekazu
Dept. Radiol., Nagoya Univ.
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FUKAMI HIROKO
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute
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KIYONO TOHRU
Division of Virology, Aichi Cancer Center Research Institute
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NAKATSUGAWA SHIGEKAZU
Department of Radiology, Nagoya University School of Medicine
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HAMAGUCHI MICHINARI
Department of Molecular Pathogenesis, Nagoya University School of Medicine
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Hamaguchi Michinari
Dept. Mol. Patho. Nagoya Univ.
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Hamaguchi M
Department Of Molecular Pathogenesis Nagoya University School Of Medicine
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Hamaguchi Michinari
Division Of Cancer Biology Nagoya University Graduate School Of Medicine
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Hamaguchi Michinari
Department Of Molecular Pathogenesis Nagoya University School Of Medicine
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Nakamura Hideaki
Department Of Analytical Chemistry Hoshi University
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Nakamura Hideaki
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden
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Ishizaki K
Aichi Cancer Center Research Institute
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Ishizaki Kanji
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Ishizaki Kanji
大阪府立大学先端科学イノベーションセンター 放射線生命科学
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Hayashi Yuko
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Kiyono Tohru
Division Of Virology Aichi Cancer Center Research Institute
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Hayashi Y
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute Kanokoden
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Fukami Hiroko
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
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Fukami Hiroko
Central Lab. And Radiat. Biol. Aichi Cancer Center Res. Inst.
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Hayashi Yasushi
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Nakatsugawa Shigekazu
Dept. Radiol. Nagoya Univ.
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Nakatsugawa Shigekazu
Department Of Radiology Nagoya University Hospital
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Nakatsugawa Shigekazo
Dept. Radiol. Nagoya Univ. Sch. Med.
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Nakamura Hajime
Gastroenterological Surgery Nagoya University Graduate School Of Medicine
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Nakamura Hideaki
Department of Analytical Chemistry, Hoshi University
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NAKAMURA Hideaki
Department of Physics, Kumamoto University
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