hTERT-Immortalized Cells Useful for Analyzing Effects of Low-Dose-Rate Radiation on Human Cells
スポンサーリンク
概要
- 論文の詳細を見る
To establish immortal human cells, we introduced the cDNA of the human telomere reverse transcriptase (hTERT) gene into skin fibroblast cells obtained from normal and ataxia telangiectasia (AT) individuals of Japanese origin. hTERT-immortalized cells retained their original characteristics and radiosensitivity except for immortalization, suggesting that these cells might be useful for analyzing the effects of radiation on human cells. hTERT-immortalized cells from a normal individual showed a greater resistance after low-dose-rate irradiation than after high-dose-rate irradiation. In contrast, cells from AT patients irradiated at a low-dose rate showed virtually the same survival as those irradiated at a high-dose rate. In hTERT-immortalized normal cells, the genetic effects of low-dose-rate radiation were quantitatively and qualitatively less severe than those of high-dose-rate radiation. In hTERT-immortalized AT cells, some fraction of DNA damage such as DNA double-strand breaks might not be repaired, and AT cells were severely affected by low-dose-rate radiation. The activation of ataxia telangiectasia mutated (ATM) protein was confirmed during low-dose-rate radiation, and may play an important role in repair of DNA damage induced by low-dose-rate radiation. This paper reviews briefly the current research at our laboratory. The hTERT-immortalized cells may be useful in determining the effects of low-dose and low-dose-rate radiation on human cells.
- 日本放射線影響学会の論文
- 2008-01-15
著者
-
NAKAMURA Hideaki
Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute
-
Nakamura Hideaki
Central Laboratory And Radiation Biology Aichi Cancer Center Research Institute
関連論文
- Dose-dependent promotion of rat forestomach carcinogenesis by combined treatment with sodium nitrite and ascorbic acid after initiation with N-methyl-N'-nitro-N-nitrosoguanidine : Possible contribution of nitric oxide-associated oxidative DNA damage
- AT Cells Show Dissimilar Hypersensitivity to Heavy-Ion and X-rays Irradiation
- A Repeated 28 Days Oral Dose Toxicity Study of Genistein in Rats, Based on the 'Enhanced OECD Test Guideline 407' for Screening Endocrine-disrupting Chemicals.(GENERAL SESSION BY POSTER PRESENTATION)(ENDOCRINE SYSTEM)
- P10-09 A Repeated 28 Days Oral Dose Toxicity Study of Methoxychlor in Rats, Based on the 'Enhanced OECD Test Guideline 407' for Screening Endocrine-disrupting Chemicals.
- A Repeated 28 Days Oral Dose Toxicity Study of Genistein in Rats, Based on the 'Enhanced OECD Test Guideline 407' for Screening Endocrine-disrupting Chemicals.(GENERAL SESSION BY POSTER PRESENTATION)(ENDOCRINE SYSTEM)
- P10-09 A Repeated 28 Days Oral Dose Toxicity Study of Methoxychlor in Rats, Based on the 'Enhanced OECD Test Guideline 407' for Screening Endocrine-disrupting Chemicals.
- AT Cells Show Dissimilar Hypersensitivity to Heavy-Ion and X-rays Irradiation
- Involvement of Lipid Peroxidation in Spontaneous Pancreatitis in WBN/Kob Rats
- Synergistic Effects of High-dose Soybean Intake with Iodine Deficiency, but Not Sulfadimethoxine or Phenobarbital, on Rat Thyroid Proliferation
- P-17 Sequential Observation of Acrolein-modified Protein and Effects of N-Acetylcysteins in Livers of Rats Treated with Carbon Tetrachloride.(Proceedings of the 27th Annual Meeting)
- P-14 Effects of antioxidants on spontaneous hepatic and renal lesions in LEC rats.(Proceedings of the 27th Annual Meeting)
- Lack of Modifying Effects of Environmental Estrogenic Compounds on the Development of Thyroid Proliferative Lesions in Male Rats Pretreated with N-Bis(2-hydroxypropyl)nitrosamine(DHPN)
- Inhibitory Effects of 1'-Acetoxychavicol Acetate on N-Nitrosobis(2-oxopropyl)-amine-induced Initiation of Cholangiocarcinogenesis in Syrian Hamsters
- 21D-03-4 Effects of Josamycin on The Spontaneous Development of Pancreatic Lesions in WBN/Kob Rats.
- Concurrent Administration of Fish Meal and Sodium Nitrite Does not Promote Glandular Stomach Carcinogenesis in Rats after Treatment with N-methyl-N'-nitro-N-nitrosoguanidine
- No Induction of p53 Phosphorylation and Few Focus Formation of Phosphorylated H2AX Suggest Efficient Repair of DNA Damage During Chronic Low-dose-rate Irradiation in Human Cells
- 28 AT cells in GI phase are sensitive to low dose rate radiation(Low dose and low dose-rate effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Genetic effect of low dose rate radiation on human cells immortalized by introducing the hTERT gene
- Establishment of Immortal Normal and Ataxia Telangiectasia Fibroblast Cell Lines by Introduction of the hTERT Gene
- Genetic Effect of Low-Dose-Rate Radiation on Human Immortal Cells
- 22D-10-1 Effects of Oltipraz on Spontaneous Hepatitis of LEC Rats.
- 21C-10-4 Effects of Combined Treatment with Antioxidants and Sodium Nitrite on the Rat Forestomach.
- INFLUENCES OF LONG-TERM ADMINISTRATION OF 24R, 25-DIHYDROXYVITAMIN D_3, A VITAMIN D_3 DERIVATIVE, IN RATS
- Effect of Clofibrate on the Erythrocyte Membrane in Rats
- Effects of Low Protein Diet on the Toxicity Induced by Potassium Bromate in Rats
- 2B-07 The Utility of Alkaline Phosphatase Isozyme Analysis in Rats for Toxicity Studies : Using an ovariectomy model
- hTERT-Immortalized Cells Useful for Analyzing Effects of Low-Dose-Rate Radiation on Human Cells
- Quantum Einstein Gravity as a Differential Geometry : General Derivation of Gauge-Fixing Term and FP-Ghost Term Based on Fibre Bundle Theory : Particles and Fields