Low transposition frequency of the medaka fish Tol2 element may be due to extranuclear localization of its transposase

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概要

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• Transposase proteins of some highly active DNA-based transposable elements, such as the maize Activator element, are known to possess nuclear localization signals (NLSs). We examined if this is also the case for the transposase of the medaka fish Tol2 element, a member of the hAT (hobo/Activator/Tam3) transposable element family, using human and mouse culture cells. Unexpectedly, the transposase-lacZ fusion protein, in which the lacZ is a location marker, was found to be present in the cytoplasm rather than in the nucleus, suggesting that the Tol2 transposase contains a signal for extranuclear localization. The same staining pattern was also observed with a fusion protein containing a 33-amino-acid region at about the center of the primary structure of the transposase. The Tol2 element might have a mechanism to control its transposition frequency that includes extranuclear localization of its transposase.

• 日本遺伝学会の論文
• 2004-04-25

著者

• Hori Hiroshi

  Division of Biological Science, Graduate School of Science, Nagoya University

• TACHIBANA Akira

  Radiat. Biol. Cent., Kyoto Univ.

• Iida Atsuo

  Division Of Biological Science Graduate School Of Science Nagoya University

• Hori Hiroshi

  Division Of Biological Science Graduate School Of Science Nagoya University

• Iida A

  National Lab. High Energy Physics Ibaraki

• Akira Tachibana

  Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive

• Tachibana Akira

  Department Of Biology Faculty Of Science Osaka City University

• Tachibana Akira

  Radiat. Biol. Cent. Kyoto Univ.

• Koga A

  Department Of Biology College Of Arts And Sciences Hiroshima University

• Koga Akihiko

  Division Of Biological Sciences Graduate School Of Science Nagoya University

• HAMADA Susumu

  Division of Biological Science, Graduate School of Science, Nagoya University

• Hamada Susumu

  Division Of Biological Science Graduate School Of Science Nagoya University

• 古賀 章彦

  Division Of Biological Science Graduate School Of Science Nagoya University

• Koga Akihiko

  Department Of Appkied Physics Osaka University

• Tachibana Akira

  Rad. Biol. Canter Kyoto Univ.

• Hori Hiroshi

  Division Of Bioengineering Graduate School Of Engineering Science Osaka University

• Tachibana Akira

  Rad. Biol. Center Kyoto Univ.

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