Purification and Characterization of Geranylgeranyl Diphosphate Synthase from Methanobacterium thermoformicicum SF-4
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概要
- 論文の詳細を見る
Geranylgeranyl diphosphate (GGPP) synthase [EC 2.5.1.29] was purified to homogeneity from Methanobacterium thermoformicicum SF-4. The enzyme was a dimeric protein consisting of two identical subunits (M_r=39,000) and catalyzed prenyl transfer reactions using isopentenyl diphosphate (K_m=30.8μM)and either dimethylallyl diphosphate (K_m=16.8μM), geranyl diphosphate (K_m=12.6μM), or farnesyl diphosphate (K_m=14.7μM) as allylic partners. During a sequential elongation, C_5→C_<10>→C_<15>→C_<20>, intermediates were accumulated with various ratios to the final product GGPP. In the presence of 0.8M KCl, GGPP synthease activity was greatly enhanced, stabilized to heat treatment at 65℃ for 30min, and protected from inhibition by p-chloromercuribenzoic acid. No other prenyltransferase synthesizing C_<20> or shorter prenyl diphosphate was observed in M. thermoformicicum SF-4. These suggest that GGPP synthease alone is important in the biosynthetic pathways to squalene and membrane polar lipids at a chain elongation stage in this strain.
- 社団法人日本農芸化学会の論文
- 1993-07-23
著者
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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TACHIBANA Akira
Department of Biological Science, Faculty of Science, Ibaraki University
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Taniguchi M
Graduate School Of Science Osaka City University
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TANIGUCHI Makoto
Department of Neurosurgery, Tokyo Metropolitan Neurological Hospital
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Tanaka Toshio
Department Of Respiratory Medicine Allergy And Rheumatic Disease Osaka University Graduate School Of
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Oi Susumu
Department of Biology, Faculty of Science, Osaka City University
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Taniguchi Makoto
Graduate School Of Science Osaka City University
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Akira Tachibana
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Department Of Biology Faculty Of Science Osaka City University
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Tachibana Akira
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Tanaka Toshio
Department Of Cardiology Matsusaka Central General Hospital
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Taniguchi Makoto
Deprtment Of Bio- And Geoscience Graduate School Of Science Osaka City University
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Oi Susumu
Department Of Biology Faculty Of Science Osaka City University
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Tachibana Akira
Rad. Biol. Canter Kyoto Univ.
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Oi S
Osaka City Univ. Osaka Jpn
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Taniguchi M
Taiyo Kagaku Co. Ltd. Mie Jpn
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Taniguchi Makoto
Department Of Biology Faculty Of Science Osaka City University
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Taniguchi Makoto
Department Of Bio- And Geoscience Gradaute School Of Science Osaka City University
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Tachibana Akira
Rad. Biol. Center Kyoto Univ.
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Tanaka Toshio
Department Of Applied Physics Faculty Of Engineering Tokyo University
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TACHIBANA Akira
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
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Tanaka Toshio
Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy
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TANIGUCHI Makoto
Department Medical Research Institute Kanazawa Medical University
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