Biosynthesis of Poly-γ-Glutamic Acid in Plants : Transient Expression of Poly-γ-Glutamate Synthetase Complex in Tobacco Leaves(PLANT BIOTECHNOLOGY)

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概要

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• Transient expression of genes coding for the poly-γ-glutamate (γPGA) synthetase system (pgs) was investigated in tobacco plants. Three genes of the pgs, pgsA, pgsB and pgsC, were separately placed under the control of the CaMV 35S promoter and introduced into tobacco leaves via Agrobacterium infection. Synthesized γPGA in plant tissues was detected immunologically with mouse anti-γPGA antiserum which specifically reacts with γPGA on a nitrocellulose membrane. Confirmation of γPGA biosynthesis in the transient expression analysis in tobacco tissue indicates that subunits of pgs complex were expressed and reassembled in a functional form.

• 社団法人日本生物工学会の論文
• 2005-10-25

著者

• Tano Tatsuo

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• 田中 孝明

  新潟大・工・機能材料

• Fukamachi K

  Department Of Molecular Toxicology Nagoya City University Graduate School Of Medical Sciences

• Tanaka T

  Graduate School Of Science Osaka City University

• Taniguchi M

  Graduate School Of Science Osaka City University

• TANIGUCHI Makoto

  Department of Neurosurgery, Tokyo Metropolitan Neurological Hospital

• Fujita K

  Graduate School Of Science Osaka City University

• Fujita K

  Tohoku Univ. Sendai Jpn

• Tanaka Toshio

  Department Of Respiratory Medicine Allergy And Rheumatic Disease Osaka University Graduate School Of

• Taniguchi Makoto

  Graduate School of Science, Osaka City University

• HIRASAWA Eiji

  Department of Biology, Faculty of Science, Osaka City University

• Fujita Kenichi

  Department of Biology and Geosciences, Graduate School of Science, Osaka City University

• Tarui Yutaka

  Department of Biology and Geoscience, Graduate School of Science, Osaka City University

• Iida Hanako

  Department of Biology and Geoscience, Graduate School of Science, Osaka City University

• Ono Eiichiro

  Plant Biotechnology Laboratory, Institute for Fundamental Research, Suntory Limited Research Center

• Miki Wataru

  Plant Biotechnology Laboratory, Institute for Fundamental Research, Suntory Limited Research Center

• Taniguchi Makoto

  Graduate School Of Science Osaka City University

• Yamaguchi Takafumi

  Department Of Bio- And Geoscience Graduate School Of Science Osaka City University

• Fujita Ken-ichi

  Graduate School Of Science Osaka City University

• Miki Wataru

  Plant Biotechnology Laboratory Institute For Fundamental Research Suntory Limited Research Center

• Tarui Y

  Graduate School Of Science Osaka City University

• Iida Hanako

  Department Of Biology And Geoscience Graduate School Of Science Osaka City University

• Ono Eiichiro

  Plant Biotechnology Laboratory Institute For Fundamental Research Suntory Limited Research Center

• Tarui Yutaka

  Department Of Biology And Geology Graduate School Of Science Osaka City University

• Hirasawa E

  Graduate School Of Science Osaka City University

• Hirasawa Eiji

  Department Of Agricultural Chemistry Kyoto University

• Tei Yuma

  Department Of Material Science Graduate School Of Science Osaka City University

• Fujita Ken-ichi

  Department Of Biology And Geosciences Graduate School Of Science Osaka City University

• Fujita Kenichi

  Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo

• Tanaka Toshio

  Department Of Cardiology Matsusaka Central General Hospital

• Taniguchi Makoto

  Deprtment Of Bio- And Geoscience Graduate School Of Science Osaka City University

• Taniguchi M

  Taiyo Kagaku Co. Ltd. Mie Jpn

• Taniguchi Makoto

  Department Of Biology Faculty Of Science Osaka City University

• Taniguchi Makoto

  Department Of Bio- And Geoscience Gradaute School Of Science Osaka City University

• Tanaka Toshio

  Department Of Biomedical Laboratory Sciences Graduate School Of Health Sciences Tokyo Medical And De

• Tanaka Toshio

  Department Of Applied Physics Faculty Of Engineering Tokyo University

• Fujita K

  Department Of Molecular Toxicology Nagoya City University Graduate School Of Medical Sciences

• Fukamachi Katsumi

  Molecular Toxicology Nagoya City University Graduate School Of Medical Sciences

• Tanaka Toshio

  Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy

• TANIGUCHI Makoto

  Department Medical Research Institute Kanazawa Medical University

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