Evaluation of Pretreatment with Pleurotus ostreatus for Enzymatic Hydrolysis of Rice Straw(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
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The effects of biological pretreatment of rice straw using four white-rot fungi (Phanerochaete chrysosporium, Trametes versicolor, Ceriporiopsis subvermispora, and Pleurotus ostreatus) were evaluated on the basis of quantitative and structural changes in the components of the pretreated rice straw as well as susceptibility to enzymatic hydrolysis. Of these white-rot fungi, P. ostreatus selectively degraded the lignin fraction of rice straw rather than the holocellulose component. When rice straw (water content of 60%) was pretreated with P. ostreatus for 60 d, the total weight loss and the degree of Klason lignin degraded were 25% and 41%, respectively. After the pretreatment, the residual amounts of cellulose and hemicellulose were 83% and 52% of those in untreated rice straw, respectively. By enzymatic hydrolysis with a commercial cellulase preparation for 48 h, 52% holocellulose and 44% cellulose in the pretreated rice straw were solubilized. The net sugar yields based on the amounts of holocellulose and cellulose of untreated rice straw were 33% for total soluble sugar from holocellulose and 32% for glucose from cellulose. The SEM observations showed that the increase in susceptibility of rice straw to enzymatic hydrolysis by pretreatment with P. ostreatus is caused by partial degradation of the lignin seal. When the content of Klason lignin was less than 15% of the total weight of the pretreated straw, enhanced degrees of enzymatic solubilization of holocellulose and cellulose fractions were observed as the content of Klason lignin decreased.
- 社団法人日本生物工学会の論文
- 2005-12-25
著者
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SUZUKI Hiroyuki
Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Taniguchi Masayuki
Department of Materials Science and Technology, Niigata University
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Watanabe Daisuke
Department of Materials Science and Technology, Niigata University
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Sakai Kenji
Department of Plant Resources, Kyushu University
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Hoshino Kazuhiro
Department of Materials Systems Engineering and Life Science, Toyama University
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Tanaka Takaaki
Department of Materials Science and Technology, Niigata University
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Tanaka T
Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Tanaka T
Hiroshima Univ. Higashi Hiroshima Jpn
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Sonomoto Kenji
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University:
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Taniguchi M
Fac. Engineering Niigata Jpn
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Taniguchi M
Department Of Material Science And Technology Faculty Of Engineering
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Taniguchi M
Department Of Materials Science And Technology Niigata University
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Taniguchi Masayuki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Taiyouji Mayumi
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Tanaka T
Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center
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Taniguchi Masayuki
Department Of Materials Science And Technology Niigata University
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Taniguchi Masayuki
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Tanaka Takaaki
Graduate School Of Science And Technology Niigata University
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Hoshino Kazuhiro
Department Of Materials Systems Engineering And Life Science Toyama University
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Tanaka Mitsuo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Sakai Kenji
Department Of Plant Resources Kyushu University
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Sakai Kenji
Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University
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Watanabe Daisuke
National Research Institute of Brewing
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Suzuki Hiroyuki
Department Of Applied Physics School Of Science And Engineering Waseda University:(present Address)h
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Watanabe Daisuke
Department Of Dermatology Aichi Medical University School Of Medicine
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Tanaka T
Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University
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Taniguchi Masayuki
Department Of Cardiology Jikei University School Of Medicine Daisan Hospital
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Hoshino Kazuhiro
Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra
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Tanaka Takaaki
Department Of Geoscience Joetsu University Of Education
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Tanaka Takaaki
Department Of Materials Science And Technology Faculty Of Engineering Niigata University
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Tanaka Takaaki
Department Of Biotechnology Faculty Of Engineering Okayama University
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Tanaka Terumichi
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Sakai Kenji
Department Of Drug Metabolism And Pharmacokinetics Nippon Boehringer Ingelheim Co. Ltd.
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Sakai Kenji
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Taniguchi Masayuki
Department Of Physics Faculty Of Science Kobe University
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Tanaka Takaaki
Department Of Bioscience & Biotechnology Faculty Of Engineering Okayama University
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Watanabe Daisuke
Department Of Dermatology Aichi Medical University
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Suzuki Hiroyuki
Department Of Animal Science Faculty Of Agriculture Hokkaido University
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Watanabe Daisuke
Department of Applied Physics, Graduate School of Engineering, Tohoku University, Aoba-yama 05, Aoba-ku, Sendai 980-8579, Japan
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Hoshino Kazuhiro
Department of Chemical and Biochemical Engineering, Toyama University
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Takahashi Masafumi
Department of Materials Science and Technology, Niigata University
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Tanaka Terumichi
Graduate School of Science and Technology, Niigata University
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