Purification and Some Properties of NADH-Dependent Sulfite Reductase from Escherichia coli Harboring Plasmid pTHS1,Which Has the S-Adenosyl-L-Methionine : Uroporphyrinogen III Methyltransferase Gene of Thiobacillus ferrooxidans
スポンサーリンク
概要
- 論文の詳細を見る
NADH-dependent sulfite reductase (NADH-SiR) was purified to an electrophoretically homogeneous state from Escherichia coli harboring plasmid pTHS1,which has the S-adenosyl-L-methionine : uroporphyrinogen III methyltransferase gene of Thiobacillus ferrooxidans. Purified NADH-SiR had an apparent molecular weight of 350,000,and showed absorption peaks characteristic of siroheme at 405 and 591 nm. The enzyme catalyzed the reduction of sulfite ion with NADH as an electron donor to hydrogen sulfide and NAD^+. However, NADPH could not be used as an electron donor. Preparations of both NADH-dependent sulfite reductase from E. coli 32-15 (pTHS1) and iron-grown T. ferrooxians were specifically activated by molybdenum ion, suggesting that the properties of NADH-SiR from E. coli 32-15 (pTHS1) are similar to those of T. ferroxidans.
- 社団法人日本生物工学会の論文
- 1995-07-25
著者
-
SUZUKI Hiroyuki
Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine
-
Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
-
Tanaka Tsuyoshi
Department of Earth and Environmental Sciences, Nagoya University
-
Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
-
Sugio Tsuyoshi
Division Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Univers
-
Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
-
MATSUGI SINJI
Department of Biological Function and Genetic Resources Sciences, Faculty of Agriculture, Okayama Un
-
Matsugi Shinji
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Tanaka Tsuyoshi
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
-
Suzuki H
Kirin Brewery Co. Ltd. Takasaki Jpn
-
Suzuki Hiroyuki
Department Of Applied Physics School Of Science And Engineering Waseda University:(present Address)h
-
Suzuki Hiroyuki
Department Of Animal Science Faculty Of Agriculture Hokkaido University
関連論文
- 精密ろ過膜とオンライン乳酸コントローラーを備えた培養システムを用いたPropionibacterium shermaniiによるビフィズス菌特異的増殖促進物質(BGS)の生産(生物工学論文賞)
- Growth-inhibition of hiochi bacteria in namazake (raw sake) by bacteriocins from lactic acid bacteria(BREWING AND FOOD TECHNOLOGY)
- Restriction Endonuclease Aor13HI from Acidiphilium organovorum 13H, a New Isoschizomer of BspMII : Purification and Characterization
- High expression of Pirh2, an E3 ligase for p27, is associated with low expression of p27 and poor prognosis in head and neck cancers
- バイオマスプラスチック製濾過膜の開発と応用 (特集 新しいバイオマス利活用技術)
- 1Mp22 米タンパク質由来ペプチドのヒト病原菌に対する抗菌作用とその機構の解明(食品科学・食品工学,一般講演)
- 1Mp21 酒造工程における乳酸菌由来バクテリオシンの火落菌に対する殺菌作用(食品科学・食品工学,一般講演)
- 非対称型デプスフィルターによる非圧縮性粒子懸濁液の精密濾過
- 食品微生物を用いた有用生理活性物質の生産
- 乳酸菌バクテリオシンを用いた清酒の火落ち防止技術の開発
- ポリ乳酸の多孔質化・複合化による機能性材料の開発(循環型社会を支えるラクテートインダストリーの新たな研究潮流)
- 1Gp07 ヒラタケによって脱リグニン処理した稲わらを用いたエタノールの生産(培養工学,一般講演)
- 1Gp08 酵素を用いた多収米の液化糖化の最適化とエタノール生産の低コスト化(培養工学,一般講演)
- 2Ip07 清酒製造工程における乳酸菌バクテリオシンの火落菌に対する増殖阻害作用(食品科学・食品工学,一般講演)
- 2Ip08 歯周病菌に対する米由来抗菌タンパク質の検索および分離同定(食品科学・食品工学,一般講演)
- 2Ip09 抗菌活性測定法の高感度化とそれを用いた植物由来抗菌タンパク質の検索(食品科学・食品工学,一般講演)
- 2Ip16 プロテアーゼ阻害成分を含む米タンパク質の品質向上用食品素材としての評価(食品科学・食品工学,一般講演)
- Symmetry Breaking in a Frustrated Heisenberg Spin System, ZnCr_2O_4 : I. Magnetic Measurements(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Conversion from Total Cavopulmonary Shunt to Fontan Circulation : Improved Cyanosis with an 11-Year Interval
- Effect of steam explosion pretreatment on treatment with Pleurotus ostreatus for the enzymatic hydrolysis of rice straw(ENVIRONMENTAL BIOTECHNOLOGY)
- BRM cocktail treatment using PSK and OK432 significantly up-regulates the migration activity in human dendritic cells without losing effective CTL induction
- 3P-1167 Porphyromonas gingivalisに対して抗菌活性を有する新規米由来タンパク質の検索と同定(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- 3P-1166 米タンパク質由来CHペプチドの口腔細菌に対する抗菌作用とその機構の解明(4b食品科学,食品工学,一般講演,醸造・食品工学,伝統の技と先端科学技術の融合)
- Role of Neurofibromin in Modulation of Expression of the Tyroshinase-Related Protein 2 Gene
- Differential Tissue-Specific Expression of Neurofibromin Isoform mRNAs in Rat^1
- Evidence for the Presence of Two Amino-Terminal Isoforms of Neurofibromin, a Gene Product Responsible for Neurofibromatosis Type 1
- Cloning and Expression of the Thiobacillus Ferroxidans 3-Isopropylmalate Dehydrogenase Gene in Escherichia coli
- Growth Inhibition of Acidiphilium Species by Organic Acids Contained in Yeast Extract
- Isolation and Characterization of Restriction Endonucleases from Acidiphilium sp. 16R and 22M
- Crossover from Magnetic to Nonmagnetic Ground State in the Kondo Alloy System Ce (Ni_Pd_x) Sn
- Magnetic Properties of New Ternary Rare Earth Compounds RPt (Pd) Sb
- Merkel Cells in the Vellus Hair Follicles of Human Facial Skin : A Study Using Confocal Laser Microscopy
- MCH-01 DEVELOPMENT OF A NOVEL METHOD FOR STRETCHING DNA FIBERS ON MICROBRIDGES FABRICATED BY SINGLE-MASK INCLINED UV LITHOGRAPHY(Micro/Nanomechatronics I,Technical Program of Oral Presentations)
- Strong Anisotropy of Transport Properties in Metamagnetic CePtAs
- Long-term rectal temperature measurements in a patient with menstrual-associated sleep disorder
- Effects of nocturnal bright light on saliva melatonin, core body temperature and sleep propensity rhythms in human subjects
- Reservoir Competence of the Vole, Clethrionomys rufocanus bedfordiae, for Borrelia garinii or Borrelia afzelii
- COMMON PATHOGENIC MECHANISM IN DEVELOPMENT PROGRESSION OF LIVER INJURY CAUSED BY NON-ALCOHOLIC OR ALCOHOLIC STEATOHEPATITIS
- Transcriptional Activation of the Melanocyte-Specific Genes by the Human Homolog of the Mouse Microphthalmia Protein
- Tristetraprolin (TTP) gene polymorphisms in patients with rheumatoid arthritis and healthy individuals
- Isolation of an Acremonium sp. Capable of Liquefying Cross-Linked Poly(γ-Glutamic Acid) Hydrogels and the Fungal Enzyme Involved in the Disruption of γ-Ray Irradiation-Mediated Cross-Linking(MISCELLANEOUS)
- FE-P6 Growth-inhibitory activity of antimicrobial peptides from rice against oral pathogenic bacteria and clarification of their action mechanism(Section IX Food Engineering)
- Purification and Some Properties of Glutathione Reductase from Iron-oxidizing Bacterium Thiobacillus ferrooxidans
- Purification and Some Properties of NADH-Dependent Sulfite Reductase from Escherichia coli Harboring Plasmid pTHS1,Which Has the S-Adenosyl-L-Methionine : Uroporphyrinogen III Methyltransferase Gene of Thiobacillus ferrooxidans
- A DNA Region That Complements on Escherichia coli cysG Mutation in Thiobacillus ferrooxidans
- NADH-dependent Sulfite Reductase Activity in the Periplasmic Space of Thiobacillus ferrooxidans
- Inhibition of Sulfur Use by Sulfite Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purificaion and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Effects of Polyamine on the Growth of Acidiphilium facilis 24R under Acidic Conditions(Microbiology & Fermentation Industry)
- The Mechanism of Copper Leaching by Intact Cells of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- The Regulation of Sulfur Use by Ferrous Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purification and Characterization of Glycerol 3-Phosphate Dehydrogenase from Two Types of Acidiphilium sp.(Biological Chemistry)
- Effect of Co^ on the GSH Uptake and Release by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Mechanism of Tetravalent Manganese Reduction with Elemental Sulfur by Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purification and Characterization of 3-Isopropylmalate Dehydrogenase from Thiobacillus thiooxidans
- Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation
- Purification and Properties of an Acidic β-Glucosidase from Acidobacterium capsulatum
- Diurnal fluctuation of time perception under 30-h sustained wakefulness
- Time estimation during nocturnal sleep in human subjects
- Effects of small dose of brotizolam on P300
- Identification of Phoslactomycin E as a Metabolite Inducing Hyphal Morphological Abnormalities in Aspergillus fumigatus IFO 5840
- Amplification of Vacuole-targeting Fungicidal Activity of Antibacterial Antibiotic Polymyxin B by Allicin, an Allyl Sulfur Compound from Garlic
- Possible Involvement of 3-Dehydroteasterone in the Conversion of Teasterone to Typhasterol in Cultured Cells of Catharanthus roseus
- Serum levels of neutrophil activation cytokines in Kawasaki disease
- Evaluation of Pretreatment with Pleurotus ostreatus for Enzymatic Hydrolysis of Rice Straw(ENVIRONMENTAL BIOTECHNOLOGY)
- 非対称型デプスフィルターによる細菌懸濁液の精密濾過
- Estimation of Collector Current Spreading in InGaAs SHBT Having 75-nm-Thick Collector
- First NMR Observation of Valence Fluctuation in Rare-Earth Compounds : ^Se NMR Studies of Temperature-Activated Valence Fluctuation in Sm_3Se_4
- Two nap sleep test : An easy objective sleepiness test
- Evidence-Based Infection Control in Thoracic Surgery
- Identification of Brassinolide, Castasterone, Typhasterol, and Teasterone from the Pollen of Lilium elegans
- Circadian fluctuation of time perception in healthy human subjects
- Comparison of OspA Serotypes for Borrelia burgdorferi Sensu Lato from Japan, Europe and North America
- Presence of Common Antigenic Epitope in Outer Surface Protein(Osp)A and OspB of Japanese Isolates Identified as Borrelia garinii
- REGULATION OF TGF-β SIGNALING AND ITS ROLES IN PROGRESSION OF TUMORS
- Regulation of TGF-β signaling and its roles in progression of tumors
- Strategies for Treatment of Acute Aortic Dissection with Involvement of Sinus of Valsalva
- Biosynthesis of Brassinosteroids in Seedlings of Catharanthus roseus, Nicotiana tabacum, and Oryza sativa
- Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw
- Changes in cuticle of scalp hair in mild acquired zinc deficiency: A study using scanning electron microscopy
- Neoplasms in three patients following Kawasaki disease
- Transforming growth factor-β signaling is differentially inhibited by Smad2D450E and Smad3D407E
- Self-Injection-Locked, Narrow-Linewidth, Flashlamp-Pumped Ti:Al_2O_3 Laser
- A Case of Terminal Deletion of the Long Arm of Chromosome 4, del(4)(q33→ter)
- Non-Kramer's Doublet Ground State in PrPtBi
- Clinical Characteristics of Patients With Kawasaki Disease and Levels of Peripheral Endothelial Progenitor Cells and Blood Monocyte Subpopulations
- Construction of a System that Simultaneously Evaluates CYP1A1 and CYP1A2 Induction in a Stable Human-derived Cell Line using a Dual Reporter Plasmid
- Time estimation during sleep relates to the amount of slow wave sleep in humans
- Thermal Dissociation of Disilenes into Silylenes
- 非対称型デプスフィルターによる細菌懸濁液の精密濾過
- Estimation of collector current spreading in InGaAs SHBT having 75-nm-thick collector
- Estimation of collector current spreading in InGaAs SHBT having 75-nm-thick collector
- Pruritic Papular Eruptions and Candidiasis Due to HIV Infection
- Acquired Reactive Perforating Collagenosis with IgA Nephropathy
- A case of diaphragmatic clear cell carcinoma in a patient with a medical history of ovarian endometriosis
- Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying
- 2Ep19 米由来ペプチドによるチロシナーゼ活性の阻害とメラニン形成の抑制(タンパク質工学/核酸工学/ペプチド工学,一般講演)
- 2Jp05 歯周病菌に対して抗菌活性を発揮する米由来タンパク質の精製と同定(食品科学・食品工学,一般講演)
- 2Ep18 米由来ペプチドのプロテアーゼ阻害活性と抗菌活性に及ぼす塩基性アミノ酸の寄与(タンパク質工学/核酸工学/ペプチド工学,一般講演)
- 2Ep17 米タンパク質由来CHペプチドの歯周病菌に対する抗菌作用機構の解明(タンパク質工学/核酸工学/ペプチド工学,一般講演)