A DNA Region That Complements on Escherichia coli cysG Mutation in Thiobacillus ferrooxidans
スポンサーリンク
概要
- 論文の詳細を見る
Thiobacillus ferrooxidans AP19-3 has a novel NADH-dependent sulfite reductase in the periplasmic space. The gene responsible for the appearance of NADH-dependent sulfite reductase activity was cloned into a vector plasmid pBR322 to give a 5.7-kb hybrid plasmid, pTHS1, which contains a 1.3-kb DNA fragment of T. ferrooxidans AP19-3. When pTHS1 was used to transform sulfite reductase deficient E. coli mutants, strain AT2455 (cysG), JM246 (cysl), and AT2427 (cysJ), it complemented only the E. coli cysG mutation. Since cysG codes for S-adenosyl-L-methionine : uroporphyrinogen III methyltransferase, the enzyme involved in siroheme synthesis, the results indicate that the DNA region that codes for S-adenosyl-L-methionine : uroporphyrinogen III methyltransferase is present in a T.ferrooxidans 1.3kb DNA fragment on pTHS1.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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SUZUKI Hiroyuki
Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine
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Tano T
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Tano Tatsuo
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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Kanao Tadayoshi
Graduate School of Natural Science and Technology, Okayama University
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Tanaka Tsuyoshi
Department of Earth and Environmental Sciences, Nagoya University
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Kanao Tadayoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Sugio Tsuyoshi
Department of Botany and Microbiology, Division of Bioscience, Graduate School of Natural Science an
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Sugio Tsuyoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Okayama University
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Tanaka Kouji
Department of Biotechnology, School of Engineering, Nagoya University
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Matsugi Shinji
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
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Matsugi Shinji
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tanaka Tsuyoshi
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Suzuki H
Kirin Brewery Co. Ltd. Takasaki Jpn
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Suzuki Hiroyuki
Department Of Applied Physics School Of Science And Engineering Waseda University:(present Address)h
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Kanao T
Graduate School Of Natural Science And Technology Okayama University
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Kanao Tadayoshi
Faculty Of Agriculture Okayama University
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Kanao Tadayoshi
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Tanaka Kouji
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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Suzuki Hiroyuki
Department Of Animal Science Faculty Of Agriculture Hokkaido University
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Tanaka Kouji
Department of Applied Chemistry, Faculty of Engineering, Okayama University
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