Production of Extracellular Bifidogenic Growth Stimulator by Anaerobic and Aerobic Cultivations of Several Propionibacterial Strains(BIOCHEMICAL ENGINEERING)

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• Production of a bifidogenic growth stimulator (BGS) by propionic acid bacteria was investigated under anaerobic and aerobic culture conditions. To measure the concentration of extracellular BGS produced by propionic acid bacteria, we evaluated the effects of bioassay conditions using Bifidobacterium longum as a test microorganism on the formation of a growth-stimulation zone. The diameter of the growth-stimulation zone was significantly affected by both the component concentrations and the pH of a bioassay medium. The optimum component concentrations and pH of a bioassay medium were one-half of the normal values and 8.5, respectively. Using the bioassay method, we can measure the concentration of BGS produced by propionic acid bacteria ranging in concentrations from 0.1 μg/l to 1 mg/l using 1,4-dihydroxy-2-naphthoic acid (DHNA) and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as standards. Of six dairy propionic acid bacterial strains tested, the four strains (Propionibacterium freudenreichii ET-3, P. shermanii PZ-3, P. acidipropionici JCM 6432, and P.jensenii JCM 6433) produced BGS at a concentration range of 4-23 mg/l under the anaerobic culture conditions. Analysis of high performance liquid chromatography (HPLC) showed that more than 70% of total BGS produced in supernatant samples was DHNA and no ACNQ was produced by the strains. The effect of oxygen supply on BGS production was investigated for the four BGS-producing strains. The aerobic conditions exerted in positive effects on BGS production by only P. acidipropionici JCM 6432. The concentration of BGS obtained in the aerobic cultivation of P. acidipropionici JCM 6432 was 1.3-fold than that in anaerobic cultivation. Different properties (BGS production as well as cell growth and glucose metabolism) occurring in response to the aerobic conditions were observed, depending on the propionic acid bacterial strain used. This paper is the first report on BGS production by propionibacterial strains except for P. freudenreichii.

• 社団法人日本生物工学会の論文
• 2007-05-25

著者

• Tano Tatsuo

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• 田中 孝明

  新潟大・工・機能材料

• Tanaka T

  Graduate School Of Science Osaka City University

• Taniguchi Masayuki

  Department of Materials Science and Technology, Niigata University

• Tanaka Takaaki

  Department of Materials Science and Technology, Niigata University

• Kouya Tomoaki

  Graduate School Of Science And Technology Niigata University

• Horiuchi Masahito

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• KOUYA Tomoaki

  Department of Materials Science and Technology, Faculty of Engineering, Niigata University

• MISAWA Katsuhiro

  Department of Materials Science and Technology, Faculty of Engineering, Niigata University

• NAKAYAMA Eri

  Department of Materials Science and Technology, Faculty of Engineering, Niigata University

• DEGUCHI Hiroyoshi

  Department of Materials Science and Technology, Faculty of Engineering, Niigata University

• Tanaka T

  Hiroshima Univ. Higashi Hiroshima Jpn

• Nakayama Eri

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Taniguchi M

  Fac. Engineering Niigata Jpn

• Taniguchi M

  Department Of Material Science And Technology Faculty Of Engineering

• Taniguchi M

  Department Of Materials Science And Technology Niigata University

• Taniguchi Masayuki

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Taiyouji Mayumi

  Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura

• Tanaka T

  Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center

• Taniguchi Masayuki

  Department Of Materials Science And Technology Niigata University

• Taniguchi Masayuki

  Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura

• Tanaka Takaaki

  Graduate School Of Science And Technology Niigata University

• Misawa Katsuhiro

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Deguchi Hiroyoshi

  Department Of Materials Science And Technology Faculty Of Engineering Niigata University

• Tanaka Mitsuo

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Taniguchi Masayuki

  Department Of Cardiology Jikei University School Of Medicine Daisan Hospital

• Tanaka Takaaki

  Department Of Geoscience Joetsu University Of Education

• Tanaka Takaaki

  Department Of Biotechnology Faculty Of Engineering Okayama University

• Taniguchi Masayuki

  Department Of Physics Faculty Of Science Kobe University

• Tanaka Takaaki

  Department Of Bioscience & Biotechnology Faculty Of Engineering Okayama University

• Kouya Tomoaki

  Department of Materials Chemistry and Bioengineering, Oyama National College of Technology

• Takahashi Masafumi

  Department of Materials Science and Technology, Niigata University

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