Characterization of Spontaneous Mutation in the ΔsoxR and SoxS Overproducing Strains of Escherichia coli

概要

論文の詳細を見る
To examine the role of the soxRS regulon in mutagenesis, we characterized the spontaneous mutations occurring in the endogenous tonB gene in the ΔsoxR strain and the SoxS overproducing strain of Escherichia coli. Neither the ΔsoxR strain nor the SoxS overproducing strain led to an enhancement or diminishment of the spontaneous mutation frequency. By DNA sequencing, we determined 50 spontaneous mutants from the ΔsoxR strains, and found that 36% were both base substitutions and IS insertions, 14% frameshifts and 10% deletions. Among the base substitutions, G:C→T:A transversions and G:C→A:T transitions predominated, followed by A:T→T:A transversions. We determined 54 spontaneous mutants from the SoxS overproducing strains, and found that 37% were IS insertions, 31% base substitutions, 17% frameshifts, 9% deletions and 6% duplications. Among the base substitutions, G:C→T:A transversions dominated, followed by A:T→T:A transversions and G:C→A:T transitions. These results were similar to those from the soxRS+ strains. Thus, it is suggested that the soxRS-regulated genes do not play a significant role in the defense against spontaneous mutagenesis.
日本放射線影響学会の論文