Rapamycin Down-Regulates Inducible Nitric Oxide Synthase by Inducing Proteasomal Degradation
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the effect of rapamycin, a specific inhibitor of the mammalian serine/threonine kinase, mammalian target of rapamycin (mTOR), on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Pretreatment of cells with rapamycin significantly inhibited LPS-induced nitrite production and the expression of iNOS protein in a dose-dependent manner. However, LPS-induced mRNA expression of iNOS and its concomitant activation of nuclear factor (NF)-κB remained unchanged by rapamycin. Intriguingly, LPS-induced nitrite production and iNOS protein expression were partially blocked at nanomolar concentrations of rapamycin, whereas phosphorylation of both p70 S6 kinase and 4E-BP1 was completely abolished. The suppression of LPS-induced iNOS expression by rapamycin was reversed by the protease inhibitor lactacystin. Furthermore, rapamycin treatment stimulated 20S proteasome activity, which was slightly elevated by LPS. Taken together, our findings strongly suggest that rapamycin down-regulates LPS-induced iNOS protein expression via proteasomal activation, as well as through inhibition of the mTOR signaling pathway.
- 日本薬学会の論文
著者
-
PARK Jong
Department of Internal Medicine, Chonnam National University Hospital
-
CHOI Wahn
College of Medicine, Konkuk University
-
LEE Eun
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of
-
LEE Jae
Department of Metallurgy, The University of Tokyo
-
YOO Jeong
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine
-
Yoo Jeong
Department Of Herbal Pharmaceutical Development Korea Institute Of Oriental Medicine
-
Choi Wahn
College Of Medicine Konkuk University
-
Jin Hye
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Ahn Seong
Division of Molecular & Life Science, Hanyang University
-
Yoon Jong
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Han Jeung-Whan
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Lee Jae
Department Of Acupuncture & Moxibustion College Of Oriental Medicine
-
Park Jong
Department Mathematics Pusan National University
-
Lee Eun
Department Of Anesthesiology And Pain Medicine Asan Medical Center University Of Ulsan College Of Me
-
Lee Jae
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Yoo Jeong
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Lee Eun
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
-
Park Jong
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University
関連論文
- Long-Term Clinical Course of Patients With Isolated Myocardial Bridge
- Effects of Bilateral Stellate Ganglion Block on Autonomic Cardiovascular Regulation
- IS-69 Strategy for massive postpartum pulmonary embolism : outcome comparison between 2 cases and review of literature(Group9 Perinatology2,International Session)
- IS-59 The study of tissue mineral concentration in pregnant women who lived in Incheon and outcome of pregnancy(Group8 Perinatology1,International Session)
- Oral Administration of Curcumin Suppresses Production of Matrix Metalloproteinase (MMP)-1 and MMP-3 to Ameliorate Collagen-Induced Arthritis : Inhibition of the PKCδ/JNK/c-Jun Pathway
- On ε-optimality theorems and ε-duality theorems for convex semidefinite optimization problems with conic constraints (非線形解析学と凸解析学の研究--RIMS研究集会報告集)
- Phase-field Model for Solidification of Ternary Alloys
- Phase-field model for solidification of ternaryalloys
- Numerical Simulation of the Critical Velocity for Particle Pushing/Engulfment Transition in Fe-C alloys Using a Phase-field Model
- Numerical simulation of the critical velocity for particlepushing/engulfment transition in Fe-C alloys using a phase-fieldmodel
- Numerical simulation of peritectic reaction using a multi-phase-fieldmodel
- Large Scale Simulation of Dendritic Growth in Pure Undercooled Melt by Phase-field Model
- Outcomes Following Use of a Modified Duran Ring Tricuspid Valve Reconstruction Procedure for Secondary Tricuspid Regurgitation
- A Case of AV Reentrant Tachycardia due to a Concealed Accessory Pathway with Retrograde Conduction Manifested by Isoproterenol
- Incidence of hip fractures in Korea
- Early Statin Therapy Within 48 Hours Decreased One-Year Major Adverse Cardiac Events in Patients With Acute Myocardial Infarction
- Cubic-Structured HfLaO for the Blocking Layer of a Charge-Trap Type Flash Memory Device
- N-Terminal Pro-B-Type Natriuretic Peptide Predicts Significant Coronary Artery Lesion in the Unstable Angina Patients With Normal Electrocardiogram, Echocardiogram, and Cardiac Enzymes
- Radical Scavenging Activity and Inhibition of Advanced Glycation End Products(AGEs) Formation of Lespedeza Cuneata(The 24th Annual Meeting of Medical and Pharmaceutical Society for WAKAN-YAKU)
- IS-55 The comparison of treatment outcome of Transobturator tape and Tension-free vaginal tape obturator as the treatment of stress urinary incontinence(Group7 Women's Health1,International Session)
- IS-95 Sexual behavior of Korean young women : preliminary study for the introducing of HPV prophylactic vaccine(Group12 Oncology4,International Session)
- Production of Single-Chain Variable Fragment Antibody (scFv) in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods(BIOCHEMICAL ENGINEERING)
- Protective activity from hydrophilic and lipophilic free radical generators of Wen-Pi-Tang and its crude drug extracts in LLC-PK_1 cells
- Usefulness of Physical Maneuvers for Prevention of Vasovagal Syncope
- Molecular Basis of the High-Affinity Activation of Skeletal Ryanodine Receptor Ca^-Release Channel by Imperatoxin A
- GM-CSF inhibits apoptosis of neural cells via regulating the expression of apoptosis-related proteins
- IS-100 Prospective study of Epidemiologic factors in HPV infections and Cervical Intraepithelial Neoplasia in Korean women(Group12 Oncology4,International Session)
- Detection of recurrent cervical intraepithelial neoplasia by HPV test after LEEP conization(Oncology 8)
- Rapamycin Down-Regulates Inducible Nitric Oxide Synthase by Inducing Proteasomal Degradation
- Prevention of urethral stricture in insertion of an inflatable penile prosthesis
- Immuno-staining with a novel Human Papapillomavirus 16 E7 antibody in various HPV 16 positive tissues(Oncology 7)
- Inhibition of Acyl-CoA:Cholesterol Acyltransferase by Isohalobacillin, a Complex of Novel Cyclic Acylpeptides Produced by Bacillus sp.A1238
- Inhibition of the Binding of Oxidized Low Density Lipoprotein to the Macrophages by Iturin C-related Compounds
- Echocardiographic Epicardial Fat Thickness and Coronary Artery Disease
- Clinical Significance of Aortic Knob Width and Calcification in Unstable Angina
- Continuous Treatment of Gas-Phase Trichloroethylene by Burkholderia cepacia G4 in a Two-Stage Continuous Stirred Tank Reactor/Trickling Biofilter System(ENVIRONMENTAL BIOTECHNOLOGY)
- Analysis of Hu-Huang-Fan Practical Hierarchical Identity-Based Encryption Scheme
- Effects of Ginseng Total Saponins on Pacemaker Currents of Interstitial Cells of Cajal from the Small Intestine of Mice(Pharmacology)
- Stabilization Effect of Zeolite on DHFR mRNA in a Wheat Germ Cell-Free Protein Synthesis System
- A Simple Regression Method for Determining the Quantity of Translated Protein in a Cell-Free Protein Synthesis System
- Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Newly Isolated Agrobacterium sp. SH-1 and GW-014 from Structurally Unrelated Single Carbon Substrates
- Unidirectional Single-Mode 532-nm Nd : YAG Laser Using Intracavity Frequency Doubling in Nonplanar Ring Geometry
- Characteristics of a Birefringence Compensation Scheme in Nd^:YAG Rods Using a Polarization Rotator and Imaging Optics
- Tsutsugamushi Myocarditis With Congestive Heart Failure and Persistent Atrial Standstill
- Removal of Hydrogen Sulfide by Sulfate-Resistant Acidithiobacillus thiooxidans AZ11(ENVIRONMENTAL BIOTECHNOLOGY)
- Rapid DNA Hybridization Analysis Using a PDMS Microfluidic Sensor and a Molecular Beacon
- Rapid Colorimetric Assay and Yeast Surface Display for Screening of Highly Functional Fungal Lignin Peroxidase
- Characterization of Spontaneous Mutation in the ΔsoxR and SoxS Overproducing Strains of Escherichia coli
- Simultaneous Removal of H_2S and NH_3 in Biofilter Inoculated with Acidithiobacillus thiooxidans TAS(Environmental Biotechnology)
- Effect of Anti-Oxidant (Carvedilol and Probucol) Loaded Stents in a Porcine Coronary Restenosis Model
- Assessing Intraocular Pressure by Rebound Tonometer in Rats with an Air-Filled Anterior Chamber
- Regional Cerebral Hyperperfusion with Ictal Dystonic Posturing : Ictal-Interictal SPECT Subtraction
- The Anti-apoptotic Action of 5-Hydroxyindole: Protection of Mitochondrial Integrity
- Predicting Diametral Creep of the Pressure Tubes in CANDU Reactors Using Fuzzy Neural Networks
- Inhibitory Effects of Calycosin Isolated from the Root of Astragalus membranaceus on Melanin Biosynthesis(Pharmacognosy)
- Effect of Methanol Extract of Zanthoxylum piperitum Leaves and of Its Compound, Protocatechuic Acid, on Hepatic Drug Metabolizing Enzymes and Lipid Peroxidation in Rats(Food & Nutrition Science)
- Absorption, Translocation, and Metabolism of Oxyfluorfen in Rice (Oryza sativa) and Barnyardgrass (Echinochloa crus-galli) in the Dark and the Light
- S-1 Absorption, Translocation, and Metabolism of Oxyfluorfen in Rice (Oryza sativa) and Barnyardgrass (Echinochloa crus-galli) in the Dark and the Light
- The Extraction of Vehicle License Plate Region Using Edge Directional Properties of Wavelet Subband
- ON ENERGY DECAY ESTIMATES FOR THE WAVE EQUATION OF KIRCHHOFF TYPE (Nonlinear Analysis and Convex Analysis)
- Gene Expression Profile in the Anterior Regeneration of the Earthworm Using Expressed Sequence Tags
- IS-113 Relationship between hormone receptor status and pathologic factors in patients with endometrial cancer(Group13 Oncology5,International Session)
- NULL CONTROLLABILLITY OF THE INTEGRODIFFERENTIAL EQUATIONS IN BANACH SPACE(Nonlinear Analysis and Convex Analysis)
- Forefront of Diagnosis and Treatment of Deep-steam Mycology in Korea-Rhinoorbitocerebral Zygomycosis
- Effect of Carbon Source on the Mycelial Growth and Exo-Biopolymer Production by Submerged Culture of Paecilomyces japonica
- Factors Related to the Prevalence of Respiratory Symptoms in Workers in a Petrochemical Complex
- Incidental detection of needle broken in transurethral needle ablation of prostate during transurethral resection of prostate
- Thermoelectric properties of highly oriented Ca_Bi_Co_4O_9 fabricated by rolling process
- Successful treatment of granuloma annulare with topical 5-aminolaevulinic acid photodynamic therapy
- Cutaneous ciliated cyst of the inguinal area in a man
- Enhanced Production of cis, cis-Muconate in a Cell-Recycle Bioreactor
- Correction of Critical Dimension Uniformity on a Wafer by Controlling Back-Surface Transmittance Distribution of a Photomask
- Reduction of Viewing-Angle Dependent Color Shift in a Reflective Type Cholesteric Liquid Crystal Color Filter
- A Case of Edematous Striae Distensae in Lupus Nephritis
- Prediction of the Transition Criterion to Annular Flow in Two-Phase Flow Pattern of Refrigerants
- Systemic Plasmacytosis : A Case which Improved with Melphalan
- Intense accumulation of ^F-FDG, not enhancement on MRI, helps to guide the surgical biopsy accurately in soft tissue tumors
- Frequency Detuning and Stabilization of a Diode Laser Using the Zeeman Shift of the Saturated Absorption Signal of Rubidium Atoms
- P1-IS-16 A case of clear cell cancer of cervix of 6-year-old girl diagnosed by shedding mass without in utero DES exposure
- Synthesis of Polythiophene Derivatives and Their Application for Electrochemical DNA Sensor
- Chemical Vapor Deposition of TiN Films from Tetrakis(ethylmethylamido)titanium and Ammonia
- Biocatalytic Production of Chiral Epichlorohydrin in Organic Solvents
- Gas Chromatograpy-Mass Spectometric Analysis and Its Application to a Screening Procedure for Novel bacterial Polyhydroxylkanoic Acids Containing Long Chain Saturated and Unsaturated Monomers
- IS-68 Doppler assessment of fetal aortic isthmus flow in twin growth discordance(Group9 Perinatology3,International Session)
- OPTIMAL CONTROL PROBLEM FOR THE NONLINEAR HYPERBOLIC SYSTEMS (Nonlinear Analysis and Convex Analysis)
- Usefulness of Computed Tomographic Pulmonary Angiography in the Risk Stratification of Acute Pulmonary Thromboembolism : Comparison With Cardiac Biomarkers
- Myocardial Infarction With Moyamoya Disease and Pituitary Gigantism in a Young Female Patient
- Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different
- 30-P2-152 Development of the Korean Version of Compliance-Questionnaire-Rheumatology in Patients with Rheumatoid Arthritis
- Gene Expression Profile in the Anterior Regeneration of the Earthworm Using Expressed Sequence Tags
- Differential Expression of Three labial Genes during Earthworm Head Regeneration
- Effect of B Vitamin Supplementation on Lactic Acid Production by Lactobacillus casei
- VASODILATING EFFECT OF CRUDE PLATYCODIN IN ANESTHETIZED DOGS
- Effect of signal strength on reproducibility of circumpapillary retinal nerve fiber layer thickness measurement and its classification by spectral-domain optical coherence tomography
- Questionnaire survey on pediatric hypertension in Japan and Korea
- Ocular Toxocariasis in Korea
- Displacement analysis of the GPS station of Sampali, Indonesia
- Prediction of the Melt Index in a High-Density Polyethylene Process
- Preparation of Agar Nanoparticles by W/O Emulsification
- The Effect of Korean Ginseng and Coptidis rhizome on Plasma Acute Phase Substances and mRNA Expression of Proinflammatory Cytokines in Spleen of Growing Chicks Following Lipopolysaccharide Injection