Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Newly Isolated Agrobacterium sp. SH-1 and GW-014 from Structurally Unrelated Single Carbon Substrates
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概要
- 論文の詳細を見る
Various isolates from activated sludge and soil samples were investigated for their ability to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), from structurally unrelated single carbon substrates. Isolated Gram-negative bacteria, Agrobacterium sp. SH-1 and GW-014,could synthesize P(3HB-co-3HV) with 3HV monomer mole percent in the range from 1.3 to 11.4 from single carbon substrate, such as glucose, xylose or sucrose. Terpolyesters of 3HB, 3HV and 4-hydroxybutyrate (4HB) were also accumulated by Agrobacterium sp. SH-1 and GW-014 in a nitrogen-free production stage with various hydroxylkanoate substrates. The structure of P(3HB-co-3HV) synthesized from glucose was confirmed by gas chromatographic-mass spectrometric (GC-MS) analysis, and its molecular weight, polydispersity index and melting temperature were determined to be 620000,1.2 and 178℃, respectively. Total polymer content up to 78% (w/w) of dry cell weight and 3HV mole percent up to 50% could be achieved by fed-batch production from glucose and propionate.
- 社団法人日本生物工学会の論文
- 1995-04-25
著者
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LEE Eun
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of
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Lee E
Department Of Food Science And Technology Kyungsung University
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Choi C
School Of Chemical Engineering College Of Engineering Seoul National University
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KANG Sang
Department of Electrical and Computer Engineering, University of Seoul
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CHOI CHA
Department of Chemical Technology, College of Engineering, Seoul National University
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Choi Cha
Department Of Chemical Technology College Of Engineeering Seoul National University
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Kang Sang
School Of Chemical Engineering College Of Engineering Seoul National University
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Kang Sang
Department Of Electrical And Computer Engineering University Of Seoul
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Kang Sang
Department Of Anatomy And Neurobiology Institute Of Health Science College Of Medicine Gyeongsang Na
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Lee Eun
Department Of Anesthesiology And Pain Medicine Asan Medical Center University Of Ulsan College Of Me
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