Stabilization Effect of Zeolite on DHFR mRNA in a Wheat Germ Cell-Free Protein Synthesis System
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概要
- 論文の詳細を見る
The effects of zeolites and monocations on the protein synthesis in a cell-free system derived from wheat germ were investigated. M type of synthetic zeolite markedly enhanced the translation efficiency. Whereas this kind of stimulatory effect of zeolite in an Escherichia coli cell-free system resulted from a change in the salt compositions of the reaction solution with the addition of zeolite, the enhancement of protein synthesis in a wheat germ cell-free system was not due to the ion exchange reaction of zeolites. From the results of mRNA stability analysis, it was found that zeolite could stabilize the mRNA in a wheat germ cell-free protein synthesis system. The stabilization of mRNA by the simple addition of zeolites is useful for the enhancement of protein synthesis in a wheat germ cell-free system, since conventional methods to improve mRNA stability, such as the addition of nuclease inhibitor, have not been effective for a wheat germ cell-free system.
- 社団法人日本生物工学会の論文
- 2000-02-25
著者
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LEE Eun
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of
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Lee E
Department Of Food Science And Technology Kyungsung University
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Choi C
School Of Chemical Engineering College Of Engineering Seoul National University
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Jung Byeong
School Of Chemical Engineering College Of Engineering Seoul National University
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Kang Sang
School of Materials Science and Engineering, Seoul National University
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JUNG GYOO
School of Chemical Engineering, College of Engineering, Seoul National University
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KIM YOU-EIL
School of Chemical Engineering, College of Engineering, Seoul National University
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CHOI CHA
School of Chemical Engineering, College of Engineering, Seoul National University
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Choi Cha
School Of Chemical Engineering College Of Engineering Seoul National University
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Kang Sang
School Of Chemical Engineering College Of Engineering Seoul National University
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Jung Gyoo
School Of Chemical Engineering College Of Engineering Seoul National University
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Kim You-eil
School Of Chemical Engineering College Of Engineering Seoul National University
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Lee Eun
Department Of Anesthesiology And Pain Medicine Asan Medical Center University Of Ulsan College Of Me
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