Split Dose Recovery Studies using Homologous Recombination Deficient Gene Knockout Chicken B Lymphocyte Cells
スポンサーリンク
概要
- 論文の詳細を見る
To understand the role of proteins involved in DSB repair modulating SLD recovery, chicken B lymphoma (DT 40) cell lines either proficient or deficient in RAD52, XRCC2, XRCC3, RAD51C and RAD51D were subjected to fractionated irradiation and their survival curves charted. Survival curves of both WT DT40 and RAD52^<-/-> cells had a big shoulder while all the other cells exhibited small shoulders. However, at the higher doses of radiation, RAD51C^<-/-> cells displayed hypersensitivity comparable to the data obtained for the homologous recombination deficient RAD54^<-/-> cells. Repair of SLD was measured as an increase in survival after a split dose irradiation with an interval of incubation between the radiation doses. All the cell lines (parental DT40 and genetic knockout cell lines viz., RAD52^<-/->, XRCC2^<-/->, XRCC3^<-/-> RAD51C^<-/-> and RAD51D^<-/->) used in this study demonstrated a typical split-dose recovery capacity with a specific peak, which varied depending on the cell type. The maximum survival of WT DT40 and RAD52^<-/-> was reached at about 1-2 hours after the first dose of radiation and then decreased to a minimum thereafter (5h). The increase in the survival peaked once again by about 8 hours. The survival trends observed in XRCC2^<-/->, XRCC3^<-/->, RAD51C^<-/-> and RAD51D^<-/-> knockout cells were also similar, except for the difference in the initial delay of a peak survival for RAD51D^<-/-> and lower survival ratios. The second phase of increase in the survival in these cell lines was much slower in XRCC2^<-/->, XRCC3^<-/->, RAD51C^<-/-> and RAD51D^<-/-> and further delayed when compared with that of RAD52^<-/-> and parental DT40 cells suggesting a dependence on their cell cycle kinetics. This study demonstrates that the participation of RAD52, XRCC2, XRCC3, RAD51C and RAD51D in the DSB repair via homologous recombination is of less importance in comparison to RAD54, as RAD54 deficient cells demonstrated complete absence of SLD recovery.
- 日本放射線影響学会の論文
- 2007-01-15
著者
-
Utsumi Hiroshi
Research Reactor Institute Kyoto University
-
TANO Kaori
Research Reactor Institute, Kyoto Univ.
-
Takeda Shunichi
Department Of Radiation Genetics Graduate School Of Medicine Kyoto University
-
Utsumi Hiroshi
Research Reactor Institute Kyoto University:health Research Foundation
-
Tano Kaori
Research Reactor Institute Kyoto University:institute Of Traditional Chinese Medicine Suzuka Univers
-
Takeda Shunichi
Department Of Radiation Genetics Faculty Of Medicine Kyoto University
-
RAO B.
Research Reactor Institute, Kyoto University
-
Utsumi Hiroshi
Research Reactor Institute
-
Rao B.
Research Reactor Institute Kyoto University:department Of Radiobiology Mahe Life Sciences Center Man
関連論文
- Disruption of the Schizosaccharomyces pombe homolog of MutY (SpMYH).
- Increased Base Change Mutations at G:C Pairs in Escherichiacoli Deficient in Endonuclease III and VIII
- Radiation sensitivity of RAD54^, KU70^ and RAD54^/KU70^ mutants of the chicken DT40 cell line.
- Screening of inhibitors specific for homologous recombination using D40 cell lines.
- Measurement of modified thymine derivatives with GC/MS
- Mutational Specificity Induced by hydrogen peroxides in hypocatalacemic mouse cell strain and in kat mutant Escherichia coli
- Changes of mutatinal spectrum of supF gene by delayed transfection after riboflavin photosensitization
- Is the Ku70 a Target of Wortmannin, Phosphatidylinositol 3-Kinase Inhibitor?
- Role of mitochondria on apoptosis induced by mAMSA
- Biological Effects of Intermediate Energy Neutrons
- X-ray Sensitive Cells, Cell Cycle Perturbations and Giant cells
- Mutational Specificity of SupF Gene Induced by Riboflavin-Photosensitization in Escherichia coli
- Formation of 8-hydroxyguanine and FapyGua in DNA by Riboflavin Mediated Photosensitization
- Mutational Specificity of the Ferrous Ion in a supF Gene of Endonuclease III/VIII Deficient Escherichia coli
- Analysis of DNA Damage and the Repair in D. radiodurans by Pulsed-Field Gel Flectrophoresis
- Cellular effect of thermal neutron capture treatment using ^B_1-para-boronophenylalanine: Lethal effect on melanoma cells with different degrees of X-ray sensitivity
- Analysis on DNA Double Strand Breakage and the Repair in D.radiodurans by Pulsed-Field Gel Electrophoresis
- S-3-2 NHEJ pathway plays an important role in low dose-rate effects (LDRE)(Biological Effects of Low Dose and Low Dose-rate Irradiations, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- Establishment of the Screening System for Spontaneous and Radiation-Induced Mutation Frequency in the Chicken DT40 Cells
- Relationship between cell killing and mutation induction by ionizing radiation in human lymphoblastoid cells
- Mutational Specificity Induced by Reactive Oxygen in Endonuclease III and VIII Deficient Escherichia coli.
- Homologous Recombination, DNA Double-Strand Repair, Required for the Repair of Sublethal Damage (Elkind Recovery).
- DNA-PK:the Major Target for Wortmannin-mediated Radiosensitization by the Inhibition of DSB Repair via NHEJ Pathway
- Wortmannin Radiosensitization and DNA DSB Repair
- DNA repai-1network for the maintenance of genome integrity in vertebrates
- Split Dose Recovery Studies using Homologous Recombination Deficient Gene Knockout Chicken B Lymphocyte Cells
- QUESTIONS IN CLASSICRADIATION BIOLOGY
- Detection of Hypoxic Cells in Murine Tumors Using the Comet Assay: Comparison with a Conventional Radiobiological Assay
- Establishment and characterization of Roberts syndrome and SC phocomelia model medaka (Oryzias latipes)