DNA Damage Recognition Proteins Localize along Heavy Ion Induced Tracks in the Cell Nucleus

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概要

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• To identify the repair dynamics involved in high linear energy transfer (LET) radiation-induced DNA damage, phospho-H2AX (γH2AX) foci formation was analyzed after cellular exposure to iron ions (Feions, 500MeVu^<-1>,200KeVμm^<-1>). The foci located at DNA damage sites were visualized using immunocytochemical methods. Since H2AX is phosphorylated at sites of radiation-induced double strand breaks (DSB), γH2AX foci were used to detect or illuminate tracks formed by DSB after exposure to various doses of ionizing radiation. Additional DSB-recognition proteins such as ATM phospho-serine 1981, DNA-PKcs phospho-threonine 2609, NBS1 phospho-serine 343 and CHK2 phospho-threonine 68 all colocalized with γH2AX at high LET radiation induced DSB. In addition, Fe-ion induced foci remained for longer times than X-radiation induced foci. These findings suggest that Fe-ion induced damage is repaired more slowly than X-radiation induced damage, possibly because Fe-ion induced damage or lesions are more complex or extensive. Antibodies for all these phosphorylated DNA DSB recognition proteins appear to be very effective for the detection and localization of DSB.

• 2008-11-16

著者

• TAKAHASHI Akihisa

  Department of Biology, School of Medicine, Nara Medical University

• MORI Eiichiro

  Department of Biology, School of Medicine, Nara Medical University

• OHNISHI Takeo

  Department of Biology, School of Medicine, Nara Medical University

• 小野 公二

  京都大学原子炉実験所 粒子線腫瘍学研究センター

• Ohnishi Ken

  Department Of Biology Nara Medical University

• Ohnishi Takeo

  姫路獨協大学 医療保健学部作業療法学科

• Ohnishi Takeo

  奈良県立医科大学 生物学

• OHNISHI Takeo

  Dept. Biol. Nara Med. Univ.

• Mori Eiichiro

  Department Of Biology School Of Medicine Nara Medical University

• Mori Eiichiro

  奈良県立医科大学 医学部生物学

• Mori Eiichiro

  奈良県立医科大学 医学部生物学教室

• YAMAKAWA Nobuhiro

  Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University

• KIRITA Tadaaki

  Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University

• OMORI Katsunori

  Department of Space Biology and Microgravity Sciences, Japan Aerospace Exploration Agency

• ISHIOKA Noriaki

  Department of Space Biology and Microgravity Sciences, Japan Aerospace Exploration Agency

• FURUSAWA Yoshiya

  Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Instit

• Yamakawa Nobuhiro

  Department Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical University

• Yamakawa Nobuhiro

  奈良県立医科大学 医学部生物学

• Yamakawa Nobuhiro

  奈良県立医科大学 生物学

• Ishioka N

  Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration

• Ishioka Noriaki

  奈良県立医科大学 生物学

• Ishioka Noriaki

  National Space Development Agency Of Japan

• Ishioka Noriaki

  京都工芸繊維大学 工芸科学部応用生物学

• Futami Yasuyuki

  茨城県立医療大学 放射線技術科学科

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• Ono Koji

  Particle Radiation Oncology Research Center Kyoto University

• Ohnishi T

  Nara Medical Univ. Nara Jpn

• OKAICHI Kumio

  Nara Med. Univ.

• Kirita T

  Department Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical University

• Ito Nobuhiko

  Laboratory Of Veterinary Radiology And Radiation Biology Department Of Veterinary Medicine Kitasato

• Ohnishi Takeo

  Department Of Biology Nara Medical University

• Omori Katsunori

  Institute Of Space And Astronautical Science Japan Aerospace Exploration Agency (jaxa)

• Omori Katsunori

  Department Of Space Biology And Microgravity Sciences And Iss Science Project Office Institute Of Sp

• Ishioka Noriaki

  Space Utilization Research Center National Space Development Agency Of Japan (nasda)

• Ishioka Noriaki

  Department Of Space Environmental Medicine Kagoshima University Graduate School Of Medical And Denta

• Takahashi Akihisa

  Department Of Biology Nara Medical University

• Ono Koji

  京都大学原子炉実験所附属粒子線腫瘍学研究センター

• Nakagawa Yosuke

  Department Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical University

• Ono K

  Radiation Oncology Research Laboratory Research Reactor Institute Kyoto University

• Ishioka Noriaki

  Department Of Molecular Life Science Tokai University School Of Medicine

• Furusawa Yoshiya

  高エネルギー加速器研究機構物質構造科学研究所

• Ohnishi Ken

  Department Of Biology Faculty Of Science Okayama University

• Ohnishi Takeshi

  Dept. Biology Nara Med. Univ.

• Takahashi A

  Department Of Biology Nara Medical University

• Kirita Tadaaki

  Department Of Oral And Maxillofacial Surgery Nara Medical University

• Takahashi Akihisa

  奈良県立医科大学 生物学

• Nakagawa Yosuke

  Departments Of Oral And Maxillofacial Surgery School Of Medicine Nara Medical Univ.

• Ohnishi Ken

  Dept. Biology Nara Med. Univ.

• Furusawa Yoshiya

  Heavy-ion Radiobiology Res. Group National Inst. Of Radiological Sciences

• Ohnishi Takeo

  Nara Medical University

• Furusawa Yoshiya

  Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.

• Furusawa Yoshiya

  Heavy-ion Radiobiol. Res. Gr. Res. Center Charged Particle Therapy Nirs.

• Ogura Koichi

  College Of Industrial Technology Nihon University

• Ohnishi Takeo

  Dept. Of Biol. Nara Medical Univ.

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