ガンマ線照射後器官培養された豚眼毛様体上皮におけるアポトーシス

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概要

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• Background. When dissection of porcine eyes from a living body results in the cessation of aqueous humor production and blood flow, programmed cell death regulated by TPR53BP2 and BCL2 genes may occur in the pigmented epithelium (PE) and non-pigmented epithelium (NPE) of the ciliary body. Blood products are subjected to gamma irradiation in order to prevent cellular damage resulting from transfusion-associated graft-versus-host disease.We investigated whether gamma irradiation influenced BCL2 or TPR53BP2 genes as well as the morphology of the porcine ciliary body. Methods. We irradiated the anterior segments of porcine eyes by using 60Co gamma-rays (20 Gy). To study BCL2 and TPR53BP2 expression, the irradiated specimens were fixed in formalin and embedded in paraffin and then incubated with mouse monoclonal anti-human BCL2 or TPR53BP2 antibody. Results. Following dissection, an imbalance in homeostasis began with positive BCL2 and TPR53BP2 expression in the edematous ciliary processes, and resulted in atrophy of the NPE. Increased BCL2 and TPR53BP2 expression were evident just after gamma irradiation. Decreased TPR53BP2 expression occurred after 8 h of incubation, and thereby suppressed apoptosis in the NPE; hence, the structure of the ciliary body that was incubated for 8 h after gamma irradiation was well preserved. Conclusions. Irradiation renders the ciliary body in enucleated porcine eyes less vulnerable to apoptosis, and thereby exerts a profound preservative effect.

• 社団法人　日本実験動物学会の論文
• 2006-07-01

著者

• OGAWA Akira

  Department of Neurosurgery, Iwate Medical University School of Medicine

• Kinashi Yuko

  Radiation Safety And Control Research Reactor Institute Kyoto University

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency (JAEA)

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• Ogawa Akira

  Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.

• Funayama Tomoo

  Microbeam Radiation Biology Group Quantum Beam Science Directorate Japan Atomic Energy Agency

• Funayama Tomoo

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• KAGAWA Yasuhiro

  The Institute of Physical and Chemical Research

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• Kobayashi Akie

  Graduate School Of Life Sciences Tohoku University

• AKEO Kiyoshi

  Akeo Eye Clinic

• AKEO Yoko

  Akeo Eye Clinic

• Hamada Nobuyuki

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Kobayashi Yasuhiko

  Microbeam Radiation Biology Group Japan Atomic Energy Agency

• Ooba Atsushi

  Graduate School Of Life Sciences Tohoku University

• Ogawa Akira

  Department Of Biochemistry Gunma University Graduate School Of Medicine

• Ogawa Ai

  Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.

• Ogawa Akira

  Department Of Applied Chemistry Graduate School Of Engineering The University Of Tokyo

• 明尾 潔

  Akeo Eye Clinic

• 舟山 知夫

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency

• 小川 晃

  Department of Research and Examination, Takasaki National Hospital

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