The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana

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概要

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• The complete amino acid sequence of pokeweed lectin-B (PL-B) has been analyzed by first sequencing seven lysylendopeptidase peptides derived from the reduced and S-pyridylethylated PL-B and then connecting them by analyzing the arginylendopeptidase peptides from the reduced and S-carboxymethylated PL-B. PL-B consists of 295 amino acid residues and two oligosaccharides linked to Asn96 and Asn139, and has a molecular mass of 34,493 Da. PL-B is composed of seven repetitive chitin-binding domains having 48-79%) sequence homology with each other. Twelve amino acid residues including eight cysteine residues in these domains are absolutely conserved in all other chitin-binding domains of plant lectins and class I chitinases. Also, it was strongly suggested that the extremely high hemagglutinating and mitogenic activities of PL-B may be ascribed to its seven-domain structure.

• 社団法人日本農芸化学会の論文
• 1997-04-23

著者

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Yamaguchi K

  Kyushu Univ. Fukuoka Jpn

• Yamaguchi K

  Sagami Chemical Res. Center Kanagawa Jpn

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Yamaguchi Ken-ichi

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Kino Mitsutaka

  Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• YURINO Noriko

  Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Ky

• Yamaguchi Kohji

  Tonen Corporation

• Kino M

  Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Ishiguro M

  Suntory Institute For Bioorganic Research

• Yurino Noriko

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Genetic Resources Technology Kyus

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

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