An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test
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概要
- 論文の詳細を見る
The epitope chimeric antigen, CepCM, composed of the 9 selected major epitope regions (two in NS3,two each in the NS4 of two genotypes, two each in the core of two genotype, and one in the core in hepatitis C virus (HCV) polypeptide), was expressed as a fusion protein of the trpE peptide in E. coli. An ELISA test using this antigen produced the same judgements with most of the panel sera as a second generation HCV screening kit. Though discrepancies were found in twelve samples (5% of the samples), further analysis revealed that eleven samples were indeterminate sera as judged by an immunoblot test. The reactivity found in several seroconversion series sera suggested that CepCM has superior reactivity to HCV infected sera than some second generation kits. These data indicated that an epitope chimeric antigen with a man-made sequence will be a excellent tool for a diagnostic test kit.
- 公益社団法人日本薬学会の論文
- 1996-10-15
著者
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Yamaguchi K
Kyushu Univ. Fukuoka Jpn
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Yagi Shintaro
Tonen Corporation
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Yagi S
Faculty Of Pharmaceutical Sciences Okayama University
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OHTSUKA Eiko
Faculty of Pharmaceutical Sciences, Hokkaido University
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Hasegawa A
College Of Pharmacy Nihon University
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Yamada Kaoru
Department Of Chemistry Graduate School Of Science Nagoya University
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Ohtsuka E
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Ohtsuka Eiko
Faculty Of Pharmaceutical Science Hokkaido University
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Yamaguchi Kohji
Tonen Corporation
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YAGI Shintaro
Diagnostics Division, Tonen Corporation
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KASHIWAKUMA Tomiko
Diagnostics Division, Tonen Corporation
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YAMAGUCHI Kenjiro
Diagnostics Division, Tonen Corporation
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CHIBA Yukie
Diagnostics Division, Tonen Corporation
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HASEGAWA Akira
Diagnostics Division, Tonen Corporation
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Chiba Yukie
Diagnostics Division Tonen Corporation
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Kashiwakuma Tomiko
Diagnostics Division Tonen Corporation
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Hasegawa Akira
Department Of Pharmacy College Of Science And Technology Nihon University
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