Structural Analyses of Sugar Chains from Ricin A-Chain Variant(Biological Chemistry)

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概要

• 論文の詳細を見る

• Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-) sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.

• 社団法人日本農芸化学会の論文
• 1990-01-23

著者

• TADA Mikiro

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Tada M

  Univ. Tokyo Tokyo Jpn

• Takagi S

  Department Of Biology Graduate School Of Science Osaka University

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Takagi S

  Okayama Univ. Okayama Jpn

• Kimura Y

  Central Research Laboratory Hitachi Ltd.

• Kimura Yoshinobu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyusyu University:(present Office)laboratory Of Bi

• KUSUOKU Hironobu

  Laboratory of Biochemistry, Faculty of Agriculture, Okayama University

• TADA Mikiro

  Laboratory of Biochemistry, Faculty of Agriculture, Okayama University

• TAKAGI Shigeaki

  Laboratory of Biochemistry, Faculty of Agriculture, Okayama University

• Kusuoku Hironobu

  Laboratory Of Biochemistry Faculty Of Agriculture Okayama University

• Kimura Yoshinobu

  Laboratory Of Bioapplied Enzymology Department Of Bioresources Chemistry Faculty Of Agriculture Okay

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