A 40-kilodalton protein with growth inhibitory activity against the adzuki bean weevil in seeds of Vigna mungo

スポンサーリンク

概要

• 論文の詳細を見る

• A proteinous factor inhibiting the growth of the adzuki bean weevil (Callosobruchus chinensis L.) was separated from the seeds of Vigna mungo L. Hepper (urd bean), by sequential fractionations with ammonium sulfate, chromatographies on DEAE-cellulose, hydroxyapatite and Q-Sepharose columns. The proteinous factor was further purified to homogeneity by chromatography with a Mono-P column. The purified factor was a 40-kDa single polypeptide, as determined by SDS-PAGE, and heat-1abile but stable over a pH range from 4 to 6. It is noteworthy that this factor showed neither α-amylase and protease inhibitor activities nor lectin activity, in contrast to other growth inhibitors with those activities against bean weevils. The growth inhibitory activity was lost by respective digestions with α-chymotrypsin and subtilisin, indicating that the 40-kDa protein was a novel growth inhibitor in urd beans against the adzuki bean weevil.

• 日本応用動物昆虫学会の論文
• 1999-02-25

著者

• Iwasaki T

  Research & Development Laboratories Agro & Specialty Chemicals Group Nippon Kayaku Co. Ltd.

• Aizono Y

  Laboratory Of Biological Chemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• Aizono Yasuo

  Lab. Of Biological Chemistry Dep. Of Biofunctional Chemistry Graduate School Of Agricultural Sci. Ko

• Aizono Yasuo

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

• Iwasaki Teruo

  Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University

• Wang Jian-Yong

  Department of Life Science, The Graduate School of Science and Technology, Kobe University

• Wang Jian-yong

  Department Of Life Science The Graduate School Of Science And Technology Kobe University

関連論文

• Characteristics of Psychrophilic Alkaline Phosphatase
• Purification and Some Characteristics of Phosphatase of a Psychrophile
• Crystal Structure of Cold-Active Protein-Tyrosine Phosphatase from a Psychrophile, Shewanella sp.
• Synthesis and Insecticidal Activity of Novel N-Oxydihydropyrrole Derivatives with a Substituted Spirocyclohexyl Group(Organic Chemistry)
• Synthesis and Insecticidal Activity of N-Oxydihydropyrroles : 4-Hydroxy-3-mesityl-5, 5-dimethyl Derivatives with Various Substituents at the 1-Position(Organic Chemistry)
• Molecular Cloning and Characterization of a cDNA and a Gene for Subtilisin-like Serine Proteases from Rice (Oryza sativa L.) and Arabidopsis thaliana
• Temperature-dependence of the Reconstitution of an Adzuki Bean Subtilisin Inhibitor from Its Inactive Two Fragments
• Comparative Biochemistry of Serine Proteinase Inhibitors of Five Tuberous Solanum Species(Biological Chemistry)
• Pleural sarcomatoid malignant mesothelioma consisting of histiocytoid cells
• Castration Induces Apoptosis in the Mouse Epididymis during Postnatal Development
• Pleural MALT lymphoma diagnosed on thoracoscopic resection under local anesthesia using an insulation-tipped diathermic knife
• N^G-Nitro-L-arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
• Cholinergic and Serotonergic Control of Prothoracicotropic Hormone Release during the Pupal Stage of the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
• Pulmonary suture abscess with false-positive ^F-fluorodeoxyglucose positron emission scan mimicking lung cancer recurrence
• Surgically Removed Thoracolithiasis : Report of Two Cases
• Ectopic Cervico-mediastinal Thymoma Confirmed by Flow Cytometric Analysis of Tumor-derived Lymphocytes
• Specification of Amino Acid Residues Essential for the Catalytic Reaction of Cold-active Protein-tyrosine Phosphatase of a Psychrophile, Shewanella sp.
• Cloning of Cold-active Alkaline Phosphatase Gene of a Psychrophile, Shewanella sp., and Expression of the Recombinant Enzyme(Biochemistry & Molecular Biology)
• Predictive factors for postoperative acute exacerbation of interstitial pneumonia combined with lung cancer
• A 40-kilodalton protein with growth inhibitory activity against the adzuki bean weevil in seeds of Vigna mungo
• Crystal Structure of Cold-Active Alkaline Phosphatase from the Psychrophile Shewanella sp.
• A Case of Micronodular Pneumocyte Hyperplasia Diagnosed through Surgical Resection
• Carbachol-Induced Protein Phosphorylation in the Brain-Corpus Cardiacum-Corpus Allatum Complex of the Silkworm, Bombyx mori
• The release of an insect neuropeptide hormone, bombyxin, is regulated by muscarinic transmission in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori(Lepidoptera:Bombycidae)
• Enzymatical Properties of Psychrophilic Phosphatase I^1
• Intrapulmonary neurofibroma independent of neurofibromatosis type 1
• Metastatic Serous Adenocarcinoma Arising in the Adnexa Uteri and Forming Pleural Cysts on the Diaphragmatic Pleura

もっと見る 閉じる

スポンサーリンク